中国组织工程研究2012，Vol.16Issue(15)：2741-2744,4.DOI:10.3969/j.issn.1673-8225.2012.15.018
肝细胞生长因子在肿瘤坏死因子相关凋亡诱导配体作用下对原代肝星状细胞增殖及凋亡的影响
Effect of hepatocyte growth factor on the proliferation and apoptosis of primary hepatic stellate cells under tumor necrosis factor-related apoptosis-inducing ligand
摘要
Abstract
BACKGROUND: Activated hepatic stellate cells play a key role in liver fibrosis. Research shows that hepatocyte growth factor (HGF) can promote the apoptosis of activated hepatic stellate cells and the specific mechanisms may have relationship with the apoptosis of enhanced-related apoptosis-inducing ligand (TRAIL)-induced stellate cells. OBJECTIVE: To investigate the role of HGF under the action of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) in primary hepatic stellate cells (HSCs) proliferation, apoptosis and to explore the possible mechanisms involved. METHODS: Primary HSCs of SD rats were used to recovery and passage, and were used in the experiment when the proliferation was obvious. HSCs were divided into four groups: blank control group, HSCs cultured alone; HGF group,①② 100 μg/L HGF was injected into HSCs; TRAIL group, 2 mg/L ③TRAIL was injected into HSCs; HGF+TRAIL group, the HSCs ④were prestimulated by HGF for 24 hours and then 2 mg/L TRAIL was injected into HSCs. RESULTS AND CONCLUSION: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay showed that HGF at 50- 200 μg/L and TRAIL at 0.5-1.5 mg/L had no effect on HSCs proliferation. 2 mg/L rate TRAIL could inhibit HSCs proliferation. Mid and late apoptosis of HSCs was detected by flow cytometry, the apoptosis rate in the HGF+TRAIL group was higher than that in the blank control group and the HGF group (P < 0.05); the DR5 fluorescence intensity in the HGF+TRAIL group was higher than that in the blank control group, the HGF group and the TRAIL group (P < 0.01). Under the action of the TRAIL, HGF could promote the apoptosis of HSCs and inhibit the proliferation. The possible machanism was that HGF could increase the expression of DR5 on HSCs surface.关键词
肝星状细胞/肿瘤坏死因子相关凋亡诱导配体/肝细胞生长因子/凋亡/死亡受体5分类
医药卫生引用本文复制引用
张君红,姜海行,覃山羽,陆正峰,孟云超,宁琳,杨文..肝细胞生长因子在肿瘤坏死因子相关凋亡诱导配体作用下对原代肝星状细胞增殖及凋亡的影响[J].中国组织工程研究,2012,16(15):2741-2744,4.基金项目
广西自然科学基金资助项目(0897008) （0897008）
广西壮族自治区卫生厅自筹经费科研课题(Z2011317). （Z2011317）
