中国组织工程研究2012，Vol.16Issue(15)：2797-2800,4.DOI:10.3969/j.issn.1673-8225.2012.15.030
β淀粉样蛋白1~42诱导原代皮质神经元损伤过程中的淀粉样前体蛋白信号通路
Signal pathway of amyloid precursor protein in neuronal cell injury induced by amyloid-beta peptide 1-42
摘要
Abstract
BACKGROUND: Researches in recent years show that fibrillar amyloid-β peptide 1-42 can promote the accumulation of amyloid precursor protein in cell surface and lead to nerve injury. OBJECTIVE: To explore the role of amyloid precursor protein signal pathway in neuronal cell injury induced by amyloid-β peptide 1-42. METHODS: The cortical neurons were isolated from pregnant SD rats (17 to 18 days) and cultured. On the 7th day after cultivation, the neurons were incubated with 0 (normal control), 0.05, 0.5 and 5 mol/L fibrillar amyloid-β peptide 1-42 for 8 hours to construct cytotoxic model. Calcein content in the supernatant of neuron cell culture medium was determined by biochemical methods. The expression of amyloid precursor protein and Fe65 was detected by double-label immunofluorescence assays and western blotting. RESULTS AND CONCLUSION: Compared with the normal control group, the calcein releasing was significantly increased after incubation with different concentrations of fibrillar amyloid-β peptide 1-42 for 8 hours. The co-localization and expression of amyloid precursor protein and Fe65 increased according to western blotting and immunofluorescence, respectively. These findings indicate that fibrillar amyloid-β peptide 1-42 can induce toxic injury in primary cultured cortical neurons, and the amyloid precursor protein-Fe65 signal pathway may be one of the mechanisms of cytotoxic activity.关键词
淀粉样前体蛋白/β/淀粉样蛋白/神经元/毒性损伤/Fe65分类
医药卫生引用本文复制引用
邢三丽,陈川,申定珠..β淀粉样蛋白1~42诱导原代皮质神经元损伤过程中的淀粉样前体蛋白信号通路[J].中国组织工程研究,2012,16(15):2797-2800,4.基金项目
上海市教育委员会创新项目(09YZ118),课题名称:细胞表面ATP 合酶介导β-淀粉样蛋白神经毒性的机制研究. （09YZ118）
