中国组织工程研究2012，Vol.16Issue(15)：2815-2819,5.DOI:10.3969/j.issn.1673-8225.2012.15.034
尿酸钠致急性痛风性关节炎模型大鼠与槲皮素的抗炎作用
Effect of quercetin on monosodium urate crystal-induced acute inflammation in gouty arthritis Rats
黄敬群 1孙文娟 2王四旺 1朱妙章 3李舒烨1
作者信息
- 1. 解放军第四军医大学药学系天然药物学教研室,陕西省西安市,710032
- 2. 吉林医药学院药学系,吉林省吉林市,132013
- 3. 解放军第四军医大学基础部生理学教研室,陕西省西安市,710032
- 折叠
摘要
Abstract
BACKGROUND: Previous studies have shown that flavonoid quercetin has biological properties including antioxidative, anti-inflammatory and apoptosis-inducing activities. But its effect on monosodium urate crystal-induced inflammation in the rats required further investigation. OBJECTIVE: To evaluate the effect of quercetin on monosodium urate crystal-induced acute inflammation in the rats model. METHODS: Totally 72 male Sprague Dawley rats were randomly divided into six groups. Quercetin (100, 200, 400 mg/kg) and indomethacin (3.0 mg/kg) or equivalent distilled water was given orally once a day for 7 consecutive days throughout the experiment (model group and control group). At the 5th day, gouty arthritis model was established by intra-articular injection of 100 μL (3.0 mg) of monosodium urate crystal suspension inside the ankle joint of the right hind limb at 1 hour after administration. Control group was without modeling. The inflammation was quantified by measuring the circumference of the joint with a tie line method. At the 7th day, the rats were killed for sampling at 1 hour after administration. RESULTS AND CONCLUSION: The present study found that quercetin had a marked inhibitory effect on edema in the experimental gouty arthritis in a dose-dependent manner (P < 0.01), and improved the evolution of the histological signs of acute inflammation in the monosodium urate crystal arthritis in rats. In addition, the quercetin appeared to suppress neutrophil recruitment, interleukin-1β, tumor necrosis factor-α, cyclooxygenase-2 and nitric oxide by suppressing monosodium urate crystal-induced chemokine production (P < 0.05 or P < 0.01). The suppression effect was equal to indomethacin. It is clearly indicated that quercetin exerts a strong anti-inflammatory effect and can be regarded as a useful tool for the treatment of acute gouty arthritis.关键词
槲皮素/吲哚美辛/痛风性关节炎/尿酸钠/炎症因子/大鼠分类
医药卫生引用本文复制引用
黄敬群,孙文娟,王四旺,朱妙章,李舒烨..尿酸钠致急性痛风性关节炎模型大鼠与槲皮素的抗炎作用[J].中国组织工程研究,2012,16(15):2815-2819,5.
