中国组织工程研究2012，Vol.16Issue(15)：2820-2822,3.DOI:10.3969/j.issn.1673-8225.2012.15.035
三种测序DNA模板制备方法的比较
Comparison of three methods for preparing DNA sequencing templates
秦川 1张俊河 2Robert A. Mcknight 3刘世国1
作者信息
- 1. 新乡医学院分子生物学研究室,河南省新乡市453003
- 2. 新乡医学院生物化学与分子生物学教研室,河南省新乡市453003
- 3. Medicine,University,of,Utah,USA,Salt,Lake,City,84101
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摘要
Abstract
BACKGROUND: The quality of DNA templates plays a crucial role in DNA sequencing. OBJECTIVE: To provide an economical, simple method for genomic DNA sequencing and methylated DNA sequencing. METHODS: Plasmid extraction was performed using NucleoSpin multi-96 plus plasmid kit and edgebio kit SeqPrep? 96 HP plasmid prep system, respectively. Primer pairs containing target fragments were designed for the plasmids. PCR products were purified after amplification. DNA sequencing templates were prepared by the three methods above. RESULTS AND CONCLUSION: There was no significant difference in the genomic DNA sequencing results between the three methods used in the experiment (P > 0.05). As for the methylated DNA sequencing, the NucleosPin multi-96 plus plasmid kit was better than the other two methods (P < 0.05). These findings indicate that the three methods are suitable for genomic DNA sequencing, while the NucleosPin multi-96 plus plasmid kit is more situable for the methylated DNA sequencing.关键词
基因组DNA/甲基化DNA/模板/DNA/测序分类
医药卫生引用本文复制引用
秦川,张俊河,Robert A. Mcknight,刘世国..三种测序DNA模板制备方法的比较[J].中国组织工程研究,2012,16(15):2820-2822,3.
