中国组织工程研究2012，Vol.16Issue(17)：3078-3081,4.DOI:10.3969/j.issn.1673-8225.2012.17.010
激光共聚焦显微镜观察钛板和死骨表面细菌生物膜的形成能力
Formation capability of bacterial biofilm on titanium plate versus necrotic bone Characterization with confocal laser scanning microscope
摘要
Abstract
BACKGROUND: Bacteria attachment and biofilm formation at the surface of metal implants and sequestrum are major reasons for chronic infection of musculoskeletal system. OBJECTIVE: To characterize and compare the formation capability of bacterial biofilm on titanium plate and necrotic bone. METHODS: The model of bacterial biofilm was developed with modified stroma culture approach. Ten titanium plate cylinders and ten pieces of necrotic bone were randomly matched. Each pair was placed in one culture flask and immersed in bacteria solution. The biofilm of each group was stained with fluorochrome, observed and photographed using confocal laser scanning microscope. RESULTS AND CONCLUSION: Compared with the necrotic bone, the thickness of bacterial biofilm on the titanium plate was less (P < 0.05) and the percentage of live bacteria in the medium layer and the bottom layer was higher (P < 0.05). Results indicate that the bacterial biofilm tends to more easily develop on the surface of necrotic bone than on the surface of titanium plate.关键词
细菌生物膜/钛板/死骨/激光共聚焦显微镜/硬组织植入物分类
医药卫生引用本文复制引用
张志宏,孙效棠,冯安平,王万明..激光共聚焦显微镜观察钛板和死骨表面细菌生物膜的形成能力[J].中国组织工程研究,2012,16(17):3078-3081,4.基金项目
福建省自然科学基金资助(2009J05082).the Natural Science Foundation of Fujian Province,No.2009J05082 （2009J05082）
