新疆农业科学2012，Vol.49Issue(1)：113-121,9.DOI:65-1097/S.20120131.2226.018
中国美利奴羊(新疆型)及其杂交后代群体遗传多态性研究
Research on the Genetic Polymorphism of Chinese Merino (Xinjiang Type) and its Crossbred Population
摘要
Abstract
[ Objective ] To research on the genetic polymorphism about four microsatellite markers in three sheep population in order to find out genetic marker associated with growth traits and provide certain theory basis for marker assisted breeding. [ Method ] In the three sheep population ( Chinese Merino, crossbred population of a Chinese Merino and a German Merino, crossbred population of a Chinese Merino and a Suffolk) , the genetic diversity of four microsatellite markers is studied by using microsatellite marker technology. Gene frequencies (P) , gene heterozygosity ( H) , effective number of alleles(Ne) are calculated by Popgene software ( Versionl. 32 ) , and polymorphism information content ( PIC ) is calculated by PIC -CALC software. [Result]The results showed that in the three sheep population, there are 26 alleles in four microsatellite markers,and on average there are 6. 5 alleles for every microsatellite marker. Average effectivenumber of alleles(Ne) respectively is 4. 384 8, 5. 452 0, 5. 006 8; average gene heterozygosity ( H ) respectively is 0.771 3, 0.812 2, 0.799 6; average polymorphism information content(PIC) respectively is 0.742 2, 0. 785 3, 0. 770 6. [ Conclusion] It is shown that the four microsatellite markers belong to high polymorphism in the three sheep population belong on high polymorphism and they can be used as an assessment with sheep' s genetic diversity and regarded as genetic marker of growthtraits choice.关键词
中国美利奴羊(新疆型)/微卫星标记/遗传多态性Key words
Chinese Merino (Xinjiang) /microsatellite markers/genetic polymorphism分类
农业科技引用本文复制引用
秦崇凯,杨菊清,王琼,耿明,古丽尼沙·吐拉甫,刘武军..中国美利奴羊(新疆型)及其杂交后代群体遗传多态性研究[J].新疆农业科学,2012,49(1):113-121,9.基金项目
新疆维吾尔自治区高新技术研究发展计划项目(201111115) （201111115）
