新疆农业科学2012，Vol.49Issue(1)：127-132,6.DOI:65-1097/S.20120131.2231.020
健康犊牛直肠细菌的多样性
Analysis of Microbial Diversity in Rectum of Health Calves
摘要
Abstract
[ Objective ] To analyze the diversity of bacterial community in rectum in healthy calves. [Methods] 16S rRNA clone libraries were constructed, and positive clones were digested by Msp I and Hha I respectively for RFLP, and then a phylogenetic tree was depicted based on the 16S rRNA sequencing, to confirm the composition of microbes in the healthy calf rectum. [Result] The positive rate of clone was 96. 45% (488/506) , OTUs of Msp I were 64, Hha I were 45, and a total of 143, and the result revealed that the dominant position of healthy calves included Clostridium (13%), Bifidobacterium (8%), Bacteroides (3%), Lactobacillus (2%), Prevotella (2%), Eubacterium (3%), Escherichia (4%), Megasphaera (5%) and Uncultured bacterium (8%). [ Conclusion ] The bacterial population diversity is complex and abundant in rectum, and many of them are obligate anaerobe, of which Clostridium, Bifidobacterium and Megasphaera were the dominant groups. The Megasphaera had speciality in 1 - 2 week - old calves.关键词
犊牛直肠/多样性/限制性片段长度多态性(RFLP)/16S rRNA序列Key words
return/ microbial diversity/ RFLP/ 16SrRNA sequence.分类
农业科技引用本文复制引用
许强,马勋,康立超,薄新文,王新华..健康犊牛直肠细菌的多样性[J].新疆农业科学,2012,49(1):127-132,6.基金项目
新疆农垦科学院科技引导计划(YYD201107) （YYD201107）
