新疆农业科学2012，Vol.49Issue(1)：146-149,4.DOI:65-1097/S.20120131.2239.023
牦牛源多杀性巴氏杆菌ompH基因原核表达及抗原性鉴定
Expression and Antigenicity Analysis of the OmpH Gene of Pasteurella multocida from Yak
摘要
Abstract
[ Objective ] The study aims to clone and express the recombinant OmpH preotein of Pasteurella multocida (Pm) from yak. [ Methods ] The deleted signal peptide OmpH fragment of Pm from yak was amplified. The prokaryotic expression plasmid of pET28a - ompH was constructed. It was transformed into BL21(DE3) and was expressed. SDS -PAGE was carried out to identify the protein, and Western blot was employed to determine the reaction of the target protein with the Pm antibody. [Result] The construction of the prokaryotic expression plasmid of pET28a - ompH was successful. The expressed protein was about 38kDa and reactive in the Western blot detection. [ Conclusion ] The successful expression of OmpH gene takes a shot at further serological detection, polyclonal antibody preparation and vaccine development.关键词
牦牛/多杀性巴氏杆菌/ompH基因/原核表达Key words
yak/ Pasteurellosis multocida/ ompH gene/ expression分类
农业科技引用本文复制引用
田亮,康立超,赵文娟,薄新文,马勋..牦牛源多杀性巴氏杆菌ompH基因原核表达及抗原性鉴定[J].新疆农业科学,2012,49(1):146-149,4.基金项目
新疆农垦科学院科技引导计划(YYD201107) （YYD201107）
新疆农垦科学院青年基金(YQJ2009-02,YQJ201110) （YQJ2009-02,YQJ201110）
