厦门大学学报（自然科学版）2012，Vol.51Issue(1)：117-120,4.
苯丙酸对蘑菇酪氨酸酶活力的抑制作用
Studies on Inhibitory Effects of Benzenepropanoic Acid on Mushroom Tyrosinase Activity
摘要
Abstract
Tyrosinase (EC 1. 14. 18. 1) is a key enzyme of melanin synthesis. Its inhibitors hold great promise for anti-browning of fruit and vegetable. During screening the inhibitors, benzenepropanoic acid was discovered as mushroom tyrosinase inhibitor. It had obvious inhibition ability and could prevent mushroom browning and retain freshness. In the present paper,the effects of benzenepropanoic acid on the monophenolase and diphenolase activity of mushroom tyrosinase, and inhibition kinetics were studied. The results showed that benzenepropanoic acid not only depressed the activity of the steady state activity but also prolonged the lag time for the monophenolase with dose-dependence. When the concentration of benzenepropanoic acid reached 10. 0 mmol/L.the enzyme activity decreased two-thirds,and the lag time prolonged from 100 s to 140 s. Benzenepropanoic acid also could inhibited the diphenolase activity of mushroom tyrosinase with dose-dependence,and the inhibitory effect was more notable, the inhibitor concentration leading to 50% activity lost (IC50) was determined to be 2. 05 mmol/L. The inhibition of benzenepropanoic acid on the diphenolase activity was reversible. The inhibition type belonged mixed,and the inhibition constants (K1 and KIS) were determined to be 1. 570 mmol/L and 3. 070 mmol/L,respectively.关键词
酪氨酸酶/苯丙酸/单酚酶活力/二酚酶活力/抑制机理Key words
tyrosinase/benzenepropanoic acid/monophenolase activity/diphenolase activity/inhibitory mechanism分类
生物科学引用本文复制引用
郑成已,郭云集,梁戈,左麒暄,杨美花,孙黎,陈清西..苯丙酸对蘑菇酪氨酸酶活力的抑制作用[J].厦门大学学报（自然科学版）,2012,51(1):117-120,4.基金项目
国家自然科学基金项目(31071611) （31071611）
国家基础科学人才培养基金项目(J1030626) （J1030626）
福建省重点科技项目(2010N5013) （2010N5013）
