畜牧兽医学报2012，Vol.43Issue(1)：29-36,8.
山羊MyoG基因启动子区序列分析与结构预测
Sequence Analysis and Structure Prediction of the Promoter Region of MyoG Gene in Goats
摘要
Abstract
In order to explore the mechanism of goat MyoG gene transcripition regulation by stu-ding the promoter region of the gene, the 5'flanking sequence of MyoG from the Bore goat genome was attained by PCR amplification, sequencing and sequence alignment. The DNA sequence of 969 bp was amplified. The content of G and C in the sequence was about 50. 6%. The sequence analysis showed that there were one TATA-box at - 24 bp and one GC-box, two CAAT-box, two E-box and one A+T-rich motif. Meanwhile, some transcription factor binding sites including HSF, ADR1, cap were detected. The 5'flanking sequence of goat MyoG was compared with cattle, wapiti, human, mouse and chicken, the similarity was between 37. 22% and 96. 85%. It indicates that the 5' flanking sequence of MyoG is comparatively conservative among different species. This work would lay the theoretical foundation for further study for expressing and regulating mechanism of MyoG gene in goat.关键词
山羊/MyoG基因/启动子区/序列分析/结构预测Key words
goat/ MyoG gene/ promoter region/ sequence analysis/ structure prediction分类
农业科技引用本文复制引用
刘铮铸,巩元芳,张文香,付志新,张传生,宋瑜,马艳华..山羊MyoG基因启动子区序列分析与结构预测[J].畜牧兽医学报,2012,43(1):29-36,8.基金项目
河北省自然科学基金(C2007000743) （C2007000743）
国家科技支撑计划(2008BADB2B04-8-4) （2008BADB2B04-8-4）
河北科技师范学院博士基金资助(2010YB006) （2010YB006）
