畜牧兽医学报2012，Vol.43Issue(1)：37-43,7.
牛MyoG基因启动子的克隆及功能的初步分析
Cloning and Preliminary Functional Analysis of Bovine MyoG Promoter
摘要
Abstract
This experiment was conducted to compare the activity of different fragments size of Wagyu MyoG promoter and investigate the potential mechanism. According to the sequence of Bovine myogenin published in GenBank, the primers were designed to amplify the promoter region of Wagyu MyoG by performing PCR. The PCR products were ligated to Pmd18-T-MyoGpro cloning vector. Positive clones were identified by restriction endonuclease and sequencing. Then the Wagyu MyoG promoter dual-luciferase reporter gene vector (Pgl3-MyoGpro) was constructed, and then was transfected into muscle derived stem cells(MDSCs) and fetal fibroblast cells of Bos traurus, and the activity of dual-luciferase reporter gene was detected. The activity and the specificity of these promoters during 166-2 125 bp were identified in MDSCs. Bioinformatics analysis showed that the promoter fragment contained one TATA-boxt fifteen E-box and may contained binding sites of transcription factors MyoD, MEF2, MEF3, MTBF, TEF1, PRDF, Spl, SRF, ERE, GRE and Oct-1. By comparing the activity of different promoter fragments and analyzing on these transcription factors, it initially indicate that these transcription factors may play an important role in regulation of promoter activity. Cloning and analyzing of the promoter region and function of MyoG lay a foundation for further research on the mechanism of regulation and expression of MyoG in Bos taunts.关键词
MyoG基因/启动子序列/转染/双报告检测/肌肉特异性/生物信息学分析Key words
MyoG gene/ promoter sequence/ transfection/ dual-luciferase reporter detection/ muscle specificity/ bioinformatics analysis分类
农业科技引用本文复制引用
王秋华,曹允考,李树峰,佟慧丽,兴孝友,李光鹏,严云勤..牛MyoG基因启动子的克隆及功能的初步分析[J].畜牧兽医学报,2012,43(1):37-43,7.基金项目
转基因生物新品种培育科技重大专项基金(2008ZX08007-002) （2008ZX08007-002）
