畜牧兽医学报2012，Vol.43Issue(1)：50-56,7.
Iloprost对猪早期胚胎体外发育的影响
Effects of Iloprost on in vitro Development of Preimplantation Porcine Embryos
摘要
Abstract
The aim of this study was to discuss the effect of PGI2 analogue iloprost on in vitro development of porcine embryos. In this study, at different times(0, 24, 48, 72 h) , iloprost with different concentrations( 0. 2, 0. 5, 1.0, 2. 0, 5. 0, 10. 0 (μmol ? L-1)was added into the porcine zygote medium 3CPZM-3) to observe the effect of iloprost on porcine IVF embryos and to screen the best additive dosage for embryo culture, blastocyst number and blastocyst cell number were counted at 156 h after fertilization. The speed of embryo fat metabolism and related genes (cox2, creb,ppard, pdk, cpt2) expression level were tested for understanding the mechanism of iloprost promoting embryonic development. The results showed that addition of 2. 0 μmol ? L-1 iloprost at 48 hours after fertilization could significantly (P<0. 05) improve the blastocyst rate (28%vsl6%, P<0. 05) and blastocyst cell number (49. 42 vs 28. 22, P<0. 05), significantly accelerate fatty acid degradation speed (P<0. 05) and promote the expression of lipid metabolism related genes(cox2, creb,ppar8, cpt2). However there's no significant change in glucose metabolism related genes (pdk) expression level. These results demonstrates that iloprost can enhance the in vitro developmental capacity of porcine embryo by promoting fatty acid degradation.关键词
PGI2/胚胎体外发育/脂代谢/猪Key words
PGI2/ embryo in vitro development/ lipid metabolism/ swine分类
农业科技引用本文复制引用
王峰,刘仲凤,史春荣,张朔,吕明,孔庆然,刘彪,刘忠华..Iloprost对猪早期胚胎体外发育的影响[J].畜牧兽医学报,2012,43(1):50-56,7.基金项目
哈尔滨市科技创新人才基金之海外留学人员基金(2010RFLXN014) （2010RFLXN014）
黑龙江省教育厅"新世纪"人才基金((1153-NCET-007) （(1153-NCET-007）
