畜牧兽医学报2012，Vol.43Issue(1)：98-104,7.
基于H蛋白基因的犬瘟热病毒遗传变异分析
Genetic Variation Analysis of the Canine Distemper Virus Based on Its H Protein Gene
摘要
Abstract
Canine distemper disease caused by canine distemper virus (CDV) infection of domestic dogs and many carnivores is a deadly infectious disease. The article discussed the genetic characteristics, evolutional situations and epidemic regulation of CDV from molecular level. In this paper, we selected H protein gene of CDV as the target gene to evaluate molecular genetics, variation and epidemic regulation by compared the sequence of wild isolates to vaccine strains,the wild isolates included 14 CDV isolates from different geographical areas of China during the years 2002 to 2010, 12 CDV isolates around the world in 2006 to 2007, 12 CDV isolates from different hosts and the attenuated vaccine strains consisted of vCh, vCon, vOnder and vLed. The results showed that the nucleotide similarities of H gene between wild isolates and vaccine strains were from 86. 2% to 92. 1%, and the similarities of the deduced amino acid of H protein were 89. 1% to 91. 9%; Only Asia-I had the N-glycosylation sites of H gene at 584 site; There were a highernon-synonymous substitution rates in 3555 encoded amino acid sequences. And it indicate that an-tigenic variation of H protein may result in reduced effectiveness of attenuated vaccine, which couldn t provide complete immune response preservation for some CDV epidemic strains.关键词
犬瘟热病毒/遗传变异/基因型Key words
canine distemper virus/ genetic and variation/ genotype分类
农业科技引用本文复制引用
郭玲,雷燕,陈世界,王成东,杨晓龙,颜其贵..基于H蛋白基因的犬瘟热病毒遗传变异分析[J].畜牧兽医学报,2012,43(1):98-104,7.基金项目
教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848) （IRT0848）
成都大熊猫繁育研究基金项目 （）
科技部公益性行业科研专项(2009424188) （2009424188）
四川省科技厅科技支撑项目(2010SZ0050) （2010SZ0050）
