摘要
Abstract
The aim of the research was to investigate the antiendotoxin of Pulsatillae Decoction (PD), the levels of NO,E-selectin and IL-8 secreted by cultured rat intestinal microvascular en-dothelial cells were determined after treatment with its active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. Endothelial cell was challenged with 1 μg ? mL-1 LPS for 3 h, then treated respectively with PD at three concentrations (1, 5 and 10 mg ? mL"1) and its seven ingredients at three concentrations (1,5 and 10 μg ? mL-1). The cells were incubated at 37 'C in a cell incubator for 21 hours. The supernatants were collected and the content of NO,E-selectin and IL-8 were analyzed by ELISA kits. The results showed that as compared with LPS control group, the NO contents were significantly decreased in the middle-and high-dose groups of PD, the middle and high-dose groups of Anemonin, the middle- and high-dose groups of Berberine and the high-dose group of Esculetin (P<0. 01 or P<0. 05); TheE-selectin contents were significantly decreased in the high-dose group of PD, the high-dose group of Anemoside B4, the high-dose group of Berberine and the middle- and high-dose groups of Jateorhizine (P<0. 01 or P<0. 05); The IL-8 contents were significantly decreased in high-dose group of PD, the middle- and high-dose groups of Aesculin and the middle- and high-dose groups of Esculetin (P<0. 01 or P<0. 05). It suggested that PD may exert beneficial therapeutic actions on systemic inflammatory response syndrome, multiple organ dysfunction syndrome and immune diseases mediated by NO, E-selectin and IL-8.关键词
白头翁汤/内毒素/一氧化氮/E-选择素/白介素-8Key words
Pulsatillae Decoction/ LPS/ NO/ E-selectin/ IL-8分类
农业科技
