畜牧兽医学报2012，Vol.43Issue(2)：204-210,7.
山羊脂肪酸合酶基因(FASN)启动子结构与功能的初步分析
Structural and Functional Analysis of Fatty Acid Synthase Gene Promoter of Goat
摘要
Abstract
The objective of this study was to analyze the structure and function of fatty acid syn-thase gene(FASN)promoter of goat, to further reveal the transcriptional regulatory mechanism of FASN gene. In this study, the promoter of goat FASN gene was obtained by PCR. Seven promoter fragments in different length were obtained by deletion and cloned into luciferase reporter gene expression vectors. Then, the vectors were transfected into goat mammary epithelial cells and MCF-7 cells, their expression activity were determined by using the dual-luciferase reporter assay system. The promoter sequence of 2 589 bp of FASN gene was obtained. The bioinformatics analysis for sequence showed that there were a TATA-box at -41 bp and an E-box at -74 bp of transcription initiation site ( + 1). Luciferase reporter assays demonstrated that the core region of the promoter was from -293 bp to -79 bp conferring basal transcriptional activity. Meanwhile, some transcriptional factor binding sites including Spl, NF-Y, USF and SREBP were identified in this core region. The results indicated that the transcriptional factors such as Spl, NF-Y, USF and SREBP might be involved in the transcriptional regulation ofFASN gene.关键词
山羊/脂肪酸合酶/启动子/山羊乳腺上皮细胞Key words
goat/ fatty acid synthase(FASN)/ promoter/ goat mammary gland epithelial cell分类
农业科技引用本文复制引用
李君,葛婷,罗军,孙雨婷,石恒波,朱江江,郝娟,赵旺生..山羊脂肪酸合酶基因(FASN)启动子结构与功能的初步分析[J].畜牧兽医学报,2012,43(2):204-210,7.基金项目
转基因生物新品种培育项目(2009ZX08009-162B) （2009ZX08009-162B）
国家自然基金项目(31072013) （31072013）
公益性行业(农业)科研专项经费项目(201103038) （农业）
