畜牧兽医学报2012，Vol.43Issue(2)：211-219,9.
雏鸭肝炎病毒侵染下肝脏消减cDNA文库的构建及差异基因筛选
Construction of a Suppression Subtractive Hybridization cDNA Library to Screen Differentially Expressed Genes from Duck Liver Infected Duck Hepatitis Virus
摘要
Abstract
This research aimed to detect and identify genes associated with duck viral hepatitis (DVH) by construction of suppression subtractive cDNA library, and explore their mechanism by function cluster analysis. Using suppression subtraction hybridization(SSH), a different expression SSH-cDNA library of 3-day-old and full-sib ducklings infected artificially by DHV was constructed and the same day-age ducklings were injected with the same quantity of saline. Of which 563 clones were sequenced, and 299 differentially expressed sequence tags (ESTs) were obtained. After dislodging the redundant cDNA sequence and clustering splicing, the software BLAST in NCBI of GenBank was used to do the nucleic acid and protein homology comparisons and functional analysis. The results showed that 70 different genes had highly homologous with ESTs(￡ value <Ce~10,match length>150 bp,match rate>80%), most of which were related to the synthesis of cell components, signal transduction and the biological control process of pathological conditions. The occurrence and development of DVH was a complex multistep process involving multiple genes. The results provide a basis for further study of the molecular mechanism ofDVH.关键词
雏鸭/雏鸭肝炎病毒/抑制性消减杂交/表达序列标签Key words
ducklings/ DHV/SSH/ESTs分类
农业科技引用本文复制引用
李秀,徐琪,张扬,毕瑜林,赵荣雪,陈昌义,段修军,陈国宏..雏鸭肝炎病毒侵染下肝脏消减cDNA文库的构建及差异基因筛选[J].畜牧兽医学报,2012,43(2):211-219,9.基金项目
江苏省属高校自然科学基础研究面上项目(07KJB230138) （07KJB230138）
扬州大学科技创新培育基金项目(2010CXJ056) （2010CXJ056）
