畜牧兽医学报2012，Vol.43Issue(2)：220-225,6.
鹅PIT-1基因启动子区转录调控的研究
Analysis of Transcription Regulation in the Promoter Region of PIT-1 Gene in Goose
摘要
Abstract
The aim of this study was to study the possible regulation mechanism of PIT-l promoter by searching for the cis-regulatory elements, trans-acting factors and basic transcription units of the transcription regulation. The goose promoter of PIT-l gene was cloned by genome walking and subcloned into the luciferase expression vector pGL3-Basic directly. A series of promoter missing mutants were constructed ( - 1 485/-1 bp, - 1 293/-1 bp, - 1 014/-1 bp, - 775/ - 1 bp,-561/-1 bp,-353/-1 bp,-206/-1 bp) , and identified by restrictive endonuclease enzyme cutting, PCR and sequencing. The recombinant plasmids were detected by Steady-Glo? LuciferaseAssay system after transient transfecting GH3 cell. This study indicated that the PIT-l gene promoter cloned had obviously promoter activity. All of them, -561/-1 bp had highest activity, and many possible transcription factor binding sites such as (PIT-1, POU3F2,myogenin and GR) were predicted. The pGL-P/T-1 promoter was constructed successfully by series missing method. The promoter cloned in the study had activity, the positive and negative regulation areas and core promoter region were found. Furthermore, the result provided the foundation for analyzing the promoter activity and transcriptional regulation mechanism.关键词
鹅/PIT-1基因/启动子/荧光素酶基因表达载体/转录调控Key words
goose/ PIT-l gene/ promoter/ luciferase reporter vector/ transcriptional regulation分类
农业科技引用本文复制引用
赵荣雪,黄正洋,陈国宏,赵文明,徐琪,段修军,董飚,孙国波,毕瑜林,李秀,张扬..鹅PIT-1基因启动子区转录调控的研究[J].畜牧兽医学报,2012,43(2):220-225,6.基金项目
现代农业产业技术体系建设专项资金(nycytx-45-04) （nycytx-45-04）
优质高产水禽新品种选育国家科技部支撑计划重大专项资金(2006 BDA01A09) （2006 BDA01A09）
国家科技支撑计划重点项目(2006BDA14 B06) （2006BDA14 B06）
江苏省属高校自然科学基础研究面上项目(07KJB230138) （07KJB230138）
