畜牧兽医学报2012，Vol.43Issue(2)：250-254,5.
鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响
Effects of Lactobacillus rhamnosus on Anti-oxidative Functions and Cytokines Secretion in Caco-2 Cells
摘要
Abstract
In this study, the effects of Lactobacillus rhamnosus on anti-oxidative and non-specific immunologic functions in Caco-2 cells were investigated. Caco-2 cells were treated by PBS(group A), E. Coli K88(group B) and L. Rhamnosus (group C), respectively. Caco-2 cells in group D were pre-incubated with L. Rhamnosus and then infected with E. Coli K88. The results showed that T-AOC concentration decreased(P<0. 01) , but SOD activity enhanced in cell culture super-natant(P<0. Odin group B. However, the adverse situation appeared in group C and, SOD activity in cell lysate increased(P<0. 05). APRIL secretion were induced(P<0. 01), however, IL-8 secretion were inhibited(P<0. 01)both in group B and group C in different degree. IL-10 production was stopped in group B, and was promoted in group C(P<0. 01). Compared with group B, A-PRIL production significantly decreased(P<0. 01), whereas levels of IL-8 (P<0. 05)and IL-10 (P<0. Odincreased in group D. These results suggested that Caco-2 cells were sensitize to respond to L. Rhamnosus by an improved response in anti-oxidative function. In addition, inflammatory response against pathogenic bacteria in enterocyte attenuated, moreover, immune response reinforced, which indicated the activities for immunoprotection and immunoadiuvant in L.rhamnosus.关键词
鼠李糖乳杆菌/大肠杆菌K88/Caco-2细胞/抗氧化/细胞因子Key words
Lactobacillus rhamnosus j Escherichia coli K88/ Caco-2 cell/ antioxidant/ cytokine分类
医药卫生引用本文复制引用
黄怡,黄琴,李卫芬,余东游,周绪霞..鼠李糖乳杆菌对Caco-2细胞抗氧化功能和细胞因子分泌的影响[J].畜牧兽医学报,2012,43(2):250-254,5.基金项目
浙江省重大科技专项(2006C12086) （2006C12086）
国家重点基础研究发展计划(2009CB118705) （2009CB118705）
博士学科点专项科研基金(20110101110101) （20110101110101）
