畜牧兽医学报2012，Vol.43Issue(2)：255-262,8.
口蹄疫病毒通用型金标检测试纸条诊断方法的建立
Development of a Rapid Gold lmmunochromatographic Strip Test for the Diagnosis of Foot-and-Mouth Disease Virus
摘要
Abstract
The aim of this study was to develop a sensitive, rapid and simple immunity colloid gold chromatographic analysis method for examination of foot-and-mouth disease, which can make the differential diagnosis with other infectious diseases. The purified anti-FMDV Asia 1 type coupling 12S antigen rabbit antibody were coupled with colloidal gold. The purified anti-FMDV A, O type coupling 12S antigen rabbit antibody and goat anti-rabbit IgG were wrapped onto nitrocellulose membrane as the test line (T line) and the control line (C line), respectively. The FMDV general diagnosis kit was then performed. The seriate results indicated that sensitivity of the test kit reached 0. 98 X 104LD50. No cross reaction was found with SVD, VS, VES antigen by cross tests. In the clinic test, a total 90 field samples were detected with the test list. The result of corresponding rate for positive and negative samples were 96. 70% and lOO/'o. These experiments demonstrated that the established gold immunochromatographic strip test kit was sensitive, specific for detecting FMDV and was potentially useful for first time and differential diagnosis.关键词
口蹄疫病毒/偶联/通用型/金标检测试纸条/12SKey words
FMDV/ Coupling/ General/ Gold immunochromatography test/ 12S分类
农业科技引用本文复制引用
任维维,梁仲,智晓莹,祁光宇,刘湘涛,才学鹏,蒋韬..口蹄疫病毒通用型金标检测试纸条诊断方法的建立[J].畜牧兽医学报,2012,43(2):255-262,8.基金项目
甘肃省科技厅资助 （）
甘肃科技重大技术专项(1002NKDA037) （1002NKDA037）
