畜牧兽医学报2012，Vol.43Issue(2)：270-274,5.
猪瘟病毒多表位基因的表达及其免疫原性分析
Expression of Compound Multi-epitope Gene of Classical Swine Fever Virus in E.coli and the Assessment of Its Immunologicity
摘要
Abstract
Genetical engineer T, B cell epitopes of Classical swine fever virus (CSFV) were developed by employing molecular biological tools, and their immunogenicity has been primarily evaluated by immunizing experimental animals. A DNA fragment which encoding a tandem repeat protein of T, B cell epitopes of CSFV were designed and chemically synthesized, and it was cloned into pGEX-6P-l vector in turn to form a recombinant plasmid pGEXBT500. A chimeric protein was obtained after transforming the pGEX-BT500 into Escherichia coli BL21 (DE3) host cell and induced by IPTG. The western blotting analysis showed that the expressed products have a good reactogenicity, which can react specially with CSF positive sera. Inoculation with 100 fig recombinant protein induced strong neutralizing antibody response in rabbits. Our study indicated that the expressed T, B epitopes can act as the carrier protein for CSFV peptide epitopes, and this recombinant protein is a potential multiepitope peptide vaccine candidate to prevent CSFV infection.关键词
猪瘟病毒/复合多表位抗原基因/融合表达/活性分析Key words
classical swine fever virus/ compound multi-epitope gene/ fusion expression/ activation analysis分类
农业科技引用本文复制引用
田宏,侯相民,吴锦艳,陈妍,尚佑军,刘湘涛..猪瘟病毒多表位基因的表达及其免疫原性分析[J].畜牧兽医学报,2012,43(2):270-274,5.基金项目
国家863高技术研究发展计划(863)资助项目(2006AA241110) （863）
