畜牧兽医学报2012，Vol.43Issue(2)：275-281,7.
猪A群轮状病毒的分离与鉴定
Isolation and Identification of Group A Porcine Rotavirus
摘要
Abstract
Porcine rotavirus can induce serious diarrhea in piglet, but most researches are on human. We successfully isolated and identified group A porcine rotavirus (GAR) virus, which make a contribution to pathogenesis, molecular biology study on porcine rotavirus. GAR were detected by RT-PCR test from different feces samples of diarrhea, those positive samples were treated by trypsin, then was inoculated to MA-104 cell line to isolate strain TM-a. After routine RT-PCR test and electron microscope observation, we sequenced and analyzed VP6, VP7 and VP8 genes of strain TM-a. Through homology analysis, we found VP6, VP7 and VP8 are, respectively, identical to Beijing human strain LL3354 from China (96. 0%), Indian human strain RMC321 (95. 1%), wild boar strain GUB-71 from Japan (97.0%). Strain TM-a have a high similarity to human and wild boar rotavirus, we infer that GAR may spread from human to animal, and there exist gene recombination.关键词
猪A群轮状病毒/分离/鉴定Key words
group A porcine rotavirus/ isolation/ identification分类
农业科技引用本文复制引用
库旭钢,张坤,刘羽茜,何启盖..猪A群轮状病毒的分离与鉴定[J].畜牧兽医学报,2012,43(2):275-281,7.基金项目
国家生猪现代产业技术体系(CARS-36) （CARS-36）
广东省中国科学院全面战略合作项目(2010B090301020) （2010B090301020）
