畜牧与兽医2012，Vol.44Issue(2)：11-14,4.
猪抗病基因Mx1第14外显子多态性与免疫性能的相关分析
Relationship between polymorphisms of the exon 14 in Mx1 genes and immune performance in pigs
摘要
Abstract
In order to analyze the relationship between Mx\ gene polymorphisms and the immune performance in Liaoning pigs, we extracted DNA from pig ear tissues. By the PCR-RFLP method, the polymorphisms of Mx\ gene were detected in Hebao and Large White pigs. ELISA was used to measure the levels of IFN-a, IL-4 and SIgA in the two populations. The analysis results showed that Mx1 gene digested by restriction endonuclease HinSl only in Hebao pigs was divided into three kinds of genotypes of AA, AB and BB, and the frequencies of genotypes were 0. 6814, 0. 0619 and 0. 2566, respectively. In addition, the ELISA data of Hebao pigs were higher than Large White as a whole, and the fluctuation range was large. The results showed that higher immunity and much more resistance genotypes of Mil exsisted in Hebao pigs than those in Large White. Our finding provided the scientific basis for the resource preservation, variety breeding and the development and utilization of Hebao pigs.关键词
种猪/Mx1基因/PCR-RFLP/ELISAKey words
swine/ Mx1 gene/ PCR-RFLP/ ELISA分类
农业科技引用本文复制引用
赵颂,朱弘焱,于百平,宋衡元,苏玉虹..猪抗病基因Mx1第14外显子多态性与免疫性能的相关分析[J].畜牧与兽医,2012,44(2):11-14,4.基金项目
辽宁省科技厅科技项目(2009408-6). （2009408-6）
