畜牧与兽医2012，Vol.44Issue(2)：14-17,4.
鹅细小病毒延边株VP1基因真核表达质粒构建及其在Vero细胞中的表达
Construction of eukaryotic expression plasmid for VP1 gene of goose parvovirus isolated from Yanbian and its expression in Vero cells
摘要
Abstract
According to the published gene sequence of goose parvovirus ( GPV) strain B in GenBank, a pair of specific primers with Xko I and BaroH I restriction enzyme sites were designed and synthesized. The VP1 gene fragment of GPV was amplified by PCR and cloned into pMD18-T simple vector. The fragment was subcloned to eukaryotic expression vector pVAXl and the recombinant plasmid pVAXl-VPl was identified by PCR, double restriction enzyme analysis and sequence analysis. Then the recombinant pVAXl-VPl was transfected to Vero cellwith Lipofectamine 2000. The transfected cells were detected by the indirect fluorescent antibody. The specific fluorescence could be observed on the cell surface. The 1 163 bp DNA fragment was amplified from RNA extracted from the Vero cell transfected with pVAXl-VPl by RT-PCR.关键词
鹅细小病毒/VP1基因/真核表达Key words
goose parvovirus/ VP1 gene/ eukaryotic expression分类
农业科技引用本文复制引用
赵文婧,鲁承,杜秋明,高建伟,申峰勇,张颖,李闯,袁娜,房靖添..鹅细小病毒延边株VP1基因真核表达质粒构建及其在Vero细胞中的表达[J].畜牧与兽医,2012,44(2):14-17,4.基金项目
吉林省牧业管理局项目(吉牧科字第200902号). （吉牧科字第200902号）
