信阳师范学院学报（自然科学版）2012，Vol.25Issue(1)：99-102,4.DOI:10.3969/j.issn.1003-0972.2012.01.022
荧光光度法结合固相萃取测定亚硝酸盐
Determination of Nitrite by Spectrofluorimetry with Solid-phase Extraction
摘要
Abstract
An efficient solid-phase extraction column was fabricated using graphene as adsorbent. Some factors affecting the extraction efficiency were studied. Fluorescent production was produced after 8-( 3' , 4' -diaminophenyl) -difluoroboradiaza-s-indacene as fluorescent probe reacted with nitrite in aqueous medium at 30 °C for IS min. A new spectrofluorimetry method was developed for the sensitive determination of nitrite after extracting nitrite derivative. The response was a linear function of concentration in the range of 0.2 -200 nmol/L. The detection limit was calculated to be 0.08 nmol/L ( RSN = 3 ). The proposed method was used to determine the content of nitrite in food samples with the recoveries of 94.0% ~ 110.4%.关键词
荧光光度法/石墨烯/亚硝酸盐/食品Key words
spectrofluorimetry/ graphene/ nitrite/ food samples分类
化学化工引用本文复制引用
黄克靖,孙俊永,吴莹莹..荧光光度法结合固相萃取测定亚硝酸盐[J].信阳师范学院学报（自然科学版）,2012,25(1):99-102,4.基金项目
河南省高校科技创新人才基金项目(2010HASTIT025) （2010HASTIT025）
河南省科技创新杰出青年基金项目(104100510020) （104100510020）
