新医学2012，Vol.43Issue(1)：19-22,4.DOI:10.3969/g.issn.0253-9802.2012.01.007
腹腔灌注化疗联合内生场热疗在胰腺癌恶性腹水中的应用研究
Intraperitoneal chemotherapy combined with internal field thermotherapy in treating malignant ascites in patients with pancreatic cancer
摘要
Abstract
Objective; To evaluate the efficacy of intraperitoneal chemotherapy combined with internal field thermotherapy in treating malignant ascites in patients with pancreatic cancer. Methods; Intraperitoneal chemotherapy was carried out on 76 pancreatic cancer patients with malignant ascites, among which 38 patients received combined internal field thermotherapy (experimental group) , while the other 38 patients received intraperitoneal chemotherapy alone ( control group). Results: In experimental group, complete response ( CR), partial response (PR), stable disease (SD) and progressive disease (PD) were observed in 8, 21, 5 and4 patients, respectively. While in control group, CR, PR, SD and PD were observed in 3, 17, 12 and 6 patients, respectively. With a median follow-up of 11. 8 months, the median-survival time of experimental group and control group was 7. 8 and 6. 2 months, respectively, with survival rate of 29% and 18% , respectively. There were significant differences in these parameters between the two groups (P < 0. 05 ). Mild adverse events were observed in either group, with no significant difference (P > 0. 05). Conclusion: Intraperitoneal chemotherapy combined with internal field thermotherapy is more effective than intraperitoneal chemotherapy alone for pancreatic cancer patients with malignant ascites,and to investigate its biological characteristic. Methods: OVCAR/DDP cell line was established by stepwise selection in increasing concentration of cisplatin. Cell growth curve and population doubling time were measured and multidrug resistance to multi-anticancer agents was evaluated by MTT assay, Protein level of P-gp was evaluated by western blotting, and was investigated to determine the biological features of OVCAR/DDP cell line. Results: Compared to OVCAR, OVCAR/DDP cell had a prolonged doubling time. The OVCAR/DDP cell was 19. 80 times more resistant to DDP than the OVCAR parent. OVCAR/DDP cell exhibited cross-resistance to adriamycin, Flucy-tosine, paclitaxel. Western blotting showed that protein level expression of P-gp was significantly increased in OVCAR/DDP. Conclusion; OVCAR/DDP shows typical multidrug resistance phenotypes and might serve as an I-deal model for exploring the new route of reversing multidrug resistance.关键词
恶性腹水/腹腔灌注化疗/胰腺癌/内生场热疗Key words
Ovarian neoplasms/ Cisplatin/ Multidrug-resistance/ Cisplatin-resistant引用本文复制引用
张念华,陈高峰,史清华,杨薇,高海利,武如通,李寿杰..腹腔灌注化疗联合内生场热疗在胰腺癌恶性腹水中的应用研究[J].新医学,2012,43(1):19-22,4.基金项目
广东省建设中医药强省科研课题(007377) （007377）
