烟草科技Issue(2)：29-32,4.
高效液相色谱法测定卷烟主流烟气中的苯并[a]芘
Determination of Benzo[a]pyrene in Mainstream Cigarette Smoke by HPLC
程倩 1张洪非 2侯宏卫 2胡清源 2唐纲岭2
作者信息
- 1. 河北中烟工业有限责任公司技术中心,石家庄市桥西区工农路360号 050051
- 2. 国家烟草质量监督检验中心,郑州高新技术产业开发区枫杨街2号 450001
- 折叠
摘要
Abstract
A high performance liquid chromatography with fluorescence detection for determining the benzo-[ajpyrene in mainstream cigarette smoke was developed. The extraction, clean up with Florisil and chromatographic conditions were tested. The Kentucky reference cigarette 3R4F and domestic Virginia type reference cigarette samples and 50 domestic cigarette brands samples were parallel tested with the developed method and national standard method. The results showed that: 1) tobacco samples were extracted more effectively with cyclohexane under ultrasonic for 40 minutes; 2) benzo[a]pyrene was separated effectively in 30 minutes by reversed-phase HPLC with an Agilent ZORBAX Eclipse PAH column under a gradient elution with acetonitrile and water as mobile phase at a flow-rate of 2.0 mL/minute; 3) the recoveries of benzo[a]pyrene ranged from 98.13% to 101.36% with the relative standard deviation (RSD) of 1.29% to 4.32%; 4) there was no significant difference between the results determined with the developed method and national standard method. This method is suitable for determining benzo[a]pyrene in mainstream cigarette smoke.关键词
卷烟/烟气/苯并[a]芘/高效液相色谱法Key words
Cigarette/ Smoke/ Benzo[a]pyrene/ HPLC分类
轻工纺织引用本文复制引用
程倩,张洪非,侯宏卫,胡清源,唐纲岭..高效液相色谱法测定卷烟主流烟气中的苯并[a]芘[J].烟草科技,2012,(2):29-32,4.
