烟草科技Issue(2)：43-46,51,5.
HPLC-MS/MS法测定家兔血液中的NNN及其代谢物
Determination of NNN and its Metabolites in Rabbit Blood with HPLC-MS/MS
摘要
Abstract
In order to accurately determine A'-nitroso nornicotine (NNN) and its metabolites in rabbit blood, a high-performance liquid chromatography/tandem mass spectrometry method was established by optimizing chromatographic conditions and mass spectrometric conditions and using NNN-d4 as an internal standard. The samples were successively pretreated by protein precipitation, separated by a Waters-HILIC column, and analyzed by tandem mass detector under multi-reaction monitoring (MRM) mode. The results showed that the recoveries of NNN and its metabolites ranged from 80% to 111% with the relative standard deviations (RSDs) of 0.5% to 8.62% and the limits of detection of 0.039 to 0.217 ng/mL. This method is simple, sensitive, rapid, and suitable for the analysis of NNN and its metabolites in blood.关键词
N-亚硝基降烟碱(NNN)/代谢物/HPLC-MS/MSKey words
A'-nitroso nornicotine (NNN)/ Metabolite/ HPLC-MS/MS分类
轻工纺织引用本文复制引用
赵贝贝,王昇,王娟,王兆宇,张建勋..HPLC-MS/MS法测定家兔血液中的NNN及其代谢物[J].烟草科技,2012,(2):43-46,51,5.基金项目
国家自然科学基金项目"改性介孔材料固相萃取/UPLC-MSn方法研究烟草特有亚硝胺在动物体内的代谢"(21175153). （21175153）
