云南农业大学学报2012，Vol.27Issue(1)：34-38,5.DOI:10.3969/j.issn.1004-390X(n).2012.01.007
不同来源绿僵菌菌株对椰心叶甲的毒力测定
The Virulence Test of Different Strains of Metarhizium anisopliae against Brontispa longissima
摘要
Abstract
The pathogenicity of eight Metarhizium anisopliae strains against larvae and adult of Brontis-pa longissima ( Cestro) were bioassayed in laboratory by spraying. A time-dose-mortality model was applied to analysis of the variation of mortality rates. The model showed that close relationship was found between the virulence and the strains and their inoculation rate. At a concentration of 1.0 × 106~1.0 × 108 conidia/mL, the mortality rates of M. Anisopliae strains SY-2, WC-1, SX-2, HK-5, QH-4, HY-4, YP-6 and SS-2 against the larvae of B. Longissima were 74% ~92%. The mortality and LT50 of the 3rd larvae of B. Longissima inoculated with SY-2 strain at 1. 0 × 108 spores/mL was 96. 7% and 3. 8 d with significant effect (P <0. 05) different from that treated HY-4, YP-6 and SS-2 (mortality rate 88. 36% ). At a concentration of 1. 0 × 106 ~ 1. 0 × 108 conidia/ mL, the mortality rates of M. Anisopliae strains SY-2, WC-1, SX-2, HK-5, OH-4, HY-5 , YP-6 and SS-2 against the a-dult of B. Longissima were 53% ~76%. The mortality and LT50 of the adult of B. Longissima inoculated with SY-2 strain at 1. 0 × 108 spores/mL was 85. 03% and 5. 4 d with significant effect (P < 0.05) SY-2, WC-1 and SX-2 significantly different from that treated SS-2. The SY-2 was found to have very strong second infection ability.关键词
金龟子绿僵菌/椰心叶甲/毒力测定Key words
Metarhizium anisopliae/Brontispa longissima (Gestro)/virulence assay分类
农业科技引用本文复制引用
张雯龙,谭志琼..不同来源绿僵菌菌株对椰心叶甲的毒力测定[J].云南农业大学学报,2012,27(1):34-38,5.基金项目
广西农业科学院基本科研业务专项(桂农科2011YM18):海南省自然科学基金项目(80518). （桂农科2011YM18）
