云南农业大学学报2012，Vol.27Issue(1)：55-59,66,6.DOI:10.3969/j.issn.1004-390X(n).2012.01.011
日粮不同能量水平对乌金猪脂肪组织Leptin和RBP4基因表达的影响
Effect of Dietary Energy Levels on Leptin and RBP4 Gene Expression in Adipose Tissue of Wujin Pigs
摘要
Abstract
The experiments were performed to identify the effect of dietary different energy levels on leptin and RBP4 gene expression in adipose tissue of Wujin pigs. Fifty-four Wujin pigs were randomly assigned into 3 groups and feed on dietary contains three different energy levels, which were low energy (DE: 11. 74 MJ/kg), middle energy ( DE: 12. 89 MJ/kg) and high energy ( DE:4. 22 MJ/kg) respectively. At the body weights of 30, 60 and 100 kg, Wujin pigs were slaughtered and subcutaneous adipose tissue were taken for analyzing leptin and RBP4 gene expression by real-time PCR, the contents of insulin and glucose in blood and carcass fat deposition percentage were detected , respectively. The results showed that feed higher dietary energy could increase carcass fat deposition percentage and blood insulin content (P < 0. 05 ) , increase leptin gene expression ( P < 0. 05) , decrease RBP4 gene expression (P < 0. 05) , but had no influence on blood glucose content( P > 0. 05 ). This suggested that higher dietary energy could promote carcass fat deposition by up-regulating leptin gene expression, down-regulating RBP4 gene expression, and through the action of insulin.关键词
乌金猪/日粮能量水平/脂肪组织/leptin/RBP4Key words
Wujin pigs/dietary energy level/adipose tissue/leptin/RBP4分类
农业科技引用本文复制引用
潘洪彬,赵素梅,王静,黄英,张曦,葛长荣,高士争..日粮不同能量水平对乌金猪脂肪组织Leptin和RBP4基因表达的影响[J].云南农业大学学报,2012,27(1):55-59,66,6.基金项目
云南省自然科学基金重点项目(2005 C0008Z) （2005 C0008Z）
云南省自然科学基金项目(2011FB052). （2011FB052）
