云南农业大学学报2012，Vol.27Issue(1)：103-107,5.DOI:10.3969/j.issn.1004-390X(n).2012.01.019
应用双向电泳技术研究苹果实生树童期和成年期叶片蛋白质的变化
The Changes of Proteins in Apple(Malus domestica Borhk.)Leaves of Juvenile and Adult Phase by Using 2-dimensional Electrophoresis Technology
摘要
Abstract
In order to investigate the qualitative or quantitative differences of proteins during phase change in apple (Malus domestica Borkh. ) hybrid seedling (Jonathan x Golden Delicious), comparisons of protein composition between juvenile and adult tissues of apple were made by two-dimensional e-lectrophoresis (2-DE) technology. The results showed that the examination of 2-DE maps derived from leaves revealed the presence of protein spots displaying a differential expression. Among these proteins, a total of 23 differentially expressed spots were detected in different phases. Out of the 23 spots, 6 proteins were found only in the juvenile leaves and hardly detected in the mature ones, in contrast, 3 spots only detected in the adult tissues. At the same time, 11 proteins were more abundant in the adult leaf tissues than the juvenile leaves and 3 spots were more strongly expressed in the juvenile tissues. So It is indicated that these qualitatively and quantitative proteins might be the phase-related proteins.关键词
苹果/蛋白质/阶段转变Key words
apple/protein/phase change分类
农业科技引用本文复制引用
曾广娟,李春敏..应用双向电泳技术研究苹果实生树童期和成年期叶片蛋白质的变化[J].云南农业大学学报,2012,27(1):103-107,5.基金项目
河北省自然科学基金项目(C2010221576) （C2010221576）
