应用化学2012，Vol.29Issue(2)：238-244,7.DOI:10.3724/SP.J.1095.2012.00116
抗癌药物4'-O-(α-L-夹竹桃糖基)柔红霉素与小牛胸腺DNA的相互作用的荧光光谱
Fluorescence Spectrometry on the Interaction of 4'-O-(α-L-oleandrosyl) daunorubicin with Calf Thymus DNA
摘要
Abstract
The interaction of 4'-O-(α-L-oleandrosyl)daunorubicin( ODNR) with calf thymus DNA(ctDNA) in Tris-HCl buffer solution (pH =7.4) was studied by fluorescence and ultraviolet absorption spectroscopies. Effects of the ionic strength, KI quenching, and the ODNR binding differences between the single stranded DNA(ssDNA) and the double stranded DNA(dsDNA) were studied. The results showed that the major binding mode between ODNR and ctDNA was intercalation. The intrinsic fluorescence of ODNR was quenched by ctDNA through static quenching procedure. The binding constants ( K) and sites ( n) at different temperatures were obtained by Scatchard equation. The predominant intermolecular forces are likely the hydrophobic and electrostatic interactions according to the thermodynamic parameters.关键词
ODNR/小牛胸腺DNA/相互作用/荧光光谱/嵌插结合Key words
ODNR/ctDNA/interaction/fluorescence spectrum/intercalative binding分类
化学化工引用本文复制引用
崔凤灵,霍瑞娜,张贵生,邢卫卫..抗癌药物4'-O-(α-L-夹竹桃糖基)柔红霉素与小牛胸腺DNA的相互作用的荧光光谱[J].应用化学,2012,29(2):238-244,7.基金项目
国家自然科学基金资助项目(30970696) （30970696）
