湖北医药学院学报2011，Vol.30Issue(6)：565-568,4.
缺氧对Müller细胞水通道蛋白4及血管内皮生长因子的影响
Effects of Hypoxia on the Expression of AQP4 and VEGF in Human Retinal Müller Cells
蔡维 1程扬 2张有顺 3陈龙 3钱航 3李国保1
作者信息
- 1. 湖北医药学院附属东风医院眼科,湖北十堰442008
- 2. 华中科技大学同济医学院附属协和医院眼科,湖北武汉430022
- 3. 湖北医药学院附属东风医院实验中心,湖北十堰442008
- 折叠
摘要
Abstract
Objective To observe the expressions of aquaporin4 (AQP4) and vascular endothelial growth factor(VEGF) mR-NA in human retinal Miiller cells cultured under hypoxia in vitro. Methods The human Muller cells were cultured using the enzymatic digestion, identified by transmission electron microscope and immunofluorescence staining. The expressions of AQP4 mRNA and VEGF mRNA in human retinal Muller cells under hypoxia induced with CoCl2 were detected by RT-PCR. Results The positive expressions of glutamine synthetase, neuroglial fiber acidic protein, ot-smooth muscle actin, intermediate filament vimentin were showed in over 95% cells under fluorescene microscope. The expressions of AQP4 mRNA and VEGF mRNA in Muller cells were both increased with a partial dose and time dependent manner,especially of VEGF. The mRNA expression of VEGF was reached peak at 12 hours and AQP4 was at 24 hours. Conclusion Both AQP4 and VEGF may have close relationship with cell edema under hypoxia and the effect of VEGF may be earlier and stronger,which demonstrated that they may play roles in edematization of hypoxic retinopathy.关键词
水通道蛋白4/视网膜/Müller细胞/缺氧/血管内皮生长因子Key words
Aquaporin4/ Retinal/ Muller cell/ Hypoxia/ VEGF分类
医药卫生引用本文复制引用
蔡维,程扬,张有顺,陈龙,钱航,李国保..缺氧对Müller细胞水通道蛋白4及血管内皮生长因子的影响[J].湖北医药学院学报,2011,30(6):565-568,4.
