诊断学理论与实践2012，Vol.11Issue(1)：57-61,5.DOI:10.3969/j.issn.1671-2870.2012.01.014
过敏性哮喘患者嗜碱粒细胞CD63和CD203c的表达
Expressions of CD63 and CD203c in basophils of allergic asthma patients
摘要
Abstract
Objective To study the expressions of CD63 and CD203c in activated basophils of patients with allergic asthma. Methods Flow cytometry (FCM) was used to measure the expressions of CD63 and CD203c in blood basophils of allergic asthma patients, non-allergic asthma patients and normal controls, and the results were compared. Results Peripheral blood basophils could be selected by using CD 123 and HLA-DR as the gate. The median fluorescence intensity (MFI) of CD63 in basophils of allergic asthma patients increased markedly when compared with that of non-allergic asthma patients and normal controls (P<0.01). After stimulated with dust mites, the median fluorescence intensity of CD203c and CD63 in basophils of allergic asthma patients increased significantly when compared with that before stimulation and that of non-allergic asthma patients and normal controls (P<0.01). The expressions of CD203c and CD63 in basophils were correlated in certain degree with the skin test and specific IgE (slgE) level. Receiver operating characteristic (ROC) curve analysis showed that the area under curve of basophils activation test (BAT) was 0.923, higher than that of slgE (0.876). Conclusions Quantification of activated basophils by detecting CD63 expression with FCM is an effective and safe in vitro method for diagnosing allergic diseases.关键词
过敏性哮喘/嗜碱粒细胞/CD63/CD203c/流式细胞术Key words
Allergic asthma/ Basophil/ CD63/ CD203c/ Flow cytometry分类
医药卫生引用本文复制引用
杨玲,李强,王树云,许以平,郭胤仕,姚迪,史桂英..过敏性哮喘患者嗜碱粒细胞CD63和CD203c的表达[J].诊断学理论与实践,2012,11(1):57-61,5.基金项目
上海交通大学医工合作课题(YG2010MS12) （YG2010MS12）
上海市卫生局课题(20114310) （20114310）
