中国病理生理杂志2012，Vol.28Issue(4)：601-607,7.DOI:10.3969/j.issn.1000-4718.2012.04.005
自噬在熊果酸诱导的人脐静脉内皮细胞损伤中的作用
Role of autophagy in ursolic acid-induced injury of human umbilical vein endothelial cells
摘要
Abstract
AIM: To investigate the role of autophagy in the injury of human umbilical vein endothelial cells (HUVECs) induced by ursolic acid (UA). METHODS; HUVECs were cultured in vitro with UA at various concentrations for 36 h and the proliferation inhibitory rate of HUVECs was determined by MTT method . The change of ultrastructure was observed under transmission electronic microscope (TEM). The autophagy was observed using fluorescent microscope by monodansylcadaverin (MDC) staining. The protein level and mRNA expression of microtubule - associated protein light chain 3 (LC3) and Beclin - 1 were detected by Western blotting and RT - PCR, respectively. Cell apoptotic rate was meas-ured by flow cytometry analysis. RESULTS: UA at various concentrations showed significantly dose - dependent inhibitory effect on the proliferation of HUVECs. Autophagy was induced in HUVECs treated with UA as detected by MDC staining and TEM. The protein level and mRNA expression of LC 3 and Beclin - 1 in HUVECs were significantly increased following the treatment with UA , which was also in a time - dependent manner. Compared with UA group , addition of 3 - methylade-nine (3 - MA) inhibited the increase in autophagic vacuoles and exacerbated the apoptosis . CONCLUSION: Autophagy shows protective effect on the proliferation inhibition of HUVECs induced by UA and the proliferation inhibition can be en -hanced by the autophagy inhibitor 3 - MA. 3 - MA may enhance the apoptotic rate of HUVECs induced by UA .关键词
熊果酸/内皮细胞/自吞噬作用/细胞凋亡Key words
Ursolic acid/Endothelial cells/Autophagy/Apoptosis分类
医药卫生引用本文复制引用
毕娟娟,何林,余音,郭倩,叶秀峰..自噬在熊果酸诱导的人脐静脉内皮细胞损伤中的作用[J].中国病理生理杂志,2012,28(4):601-607,7.基金项目
重庆市教委科技研究项目基金资助项目(No.KJ08032) （No.KJ08032）
重庆市科技攻关计划项目(No.CSCT,2008AB5118) （No.CSCT,2008AB5118）
