中国病理生理杂志2012，Vol.28Issue(4)：664-668,5.DOI:10.3969/j.issn.1000-4718.2012.04.015
热休克蛋白在结肠炎和胰腺炎时的表达特点及大麻类物质WIN和O-1602的影响
Effects of cannabinoids WIN and O-1602 on expression of HSPs in mice with colitis or acute pancreatitis
摘要
Abstract
AIM: To investigate the effects of cannabinoids WIN 55 , 212 - 2 (WIN) and 0 - 1602 on the expression of heat - shock proteins ( HSPs) , such as HSP27 , HSP60 and HSP70, in the inflammatory tissues of mice with experimental colitis or acute pancreatitis (AP). METHODS : Mouse colitis was induced by feeding the C 57/BL with 4% dextran sulfate sodium (DSS) for 7 d, and AP was induced by intraperitoneal injection of ceruline in the mice (50 μg/kg hourly, with a total of 6 times). The mice were intraperitoneally administered with WIN or 0 - 1602 for the therapeutic e-valuation by observing the following parameters : pathological changes of the tissues , plasma activity of amylase , plasma levels of IL - 6 and cytokine - induced neutrophil chemoattractant - 1 (CINC - 1) , and HSP expression in the colonic and pancreatic tissues. RESULTS: Compared with normal control mice , the colonic tissues from colitis mice and the pancreat -ic tissues from AP mice appeared obvious signs of inflammation and injury . The plasma levels of IL - 6 and CINC - 1 significantly increased in colitis mice and AP mice. In DSS group of colitis mice , HSP27 expression increased , but HSP60and HSP70 were reduced in the colonic tissues. WIN showed anti - inflammatory effects on the pathological changes of co -Ionic tissues and the plasma cytokine levels . WIN also improved the expression of HSP70 (P < 0. 05 ). In AP group, the expression of HSP27 and HSP70 in pancreatic tissues increased , and HSP60 decreased. 0 - 1602 also showed some anti -inflammatory effects on the changes of the pathological tissues and the plasma parameters , but had no obvious effects on the HSP expression (P > 0. 05). CONCLUSION: The experimental colitis and acute pancreatitis in mice were induced by feeding DSS and injection of cerulein , respectively. The changes of HSP expression were different in the inflammatory tis -sues. WIN and 0 - 1602 show anti - inflammatory effects and increase the expression of several HSPs to some extent .关键词
热休克蛋白质类/急性胰腺炎/结肠炎/大麻酚类Key words
Heat-shock proteins/Acute pancreatitis/Colitis/Cannabinoids分类
医药卫生引用本文复制引用
余良英,李永渝,李琨,冯佳燕,林旭红,冯雅静,曹明华,徐菁..热休克蛋白在结肠炎和胰腺炎时的表达特点及大麻类物质WIN和O-1602的影响[J].中国病理生理杂志,2012,28(4):664-668,5.基金项目
国家自然科学基金资助项目(No.30971168 （）
No.81141050) （）
