中国病理生理杂志2012，Vol.28Issue(4)：683-688,6.DOI:10.3969/j.issn.1000-4718.2012.04.018
Aβ1-42作用的小胶质细胞对体外培养的神经干细胞生存的影响
Effects of Aβ1-42-induced microglial cells on survival of neural stem cells in vitro
摘要
Abstract
AIM: To explore the effects of β-amyloid protein 1-42 (Aβ2-42) -induced microglia on the survival of cultured neural stem cells ( NSCs) in vitro. METHODS: Using the Transwell chambers to build a coculture system of NSCs and microglia , we detected the proliferation , differentiation and apoptosis of the NSCs with the microglia before and after induction by Aβ-42. RESULTS; Compared with non - intervention group , the proliferation rate of NSCs in Aβ1-42 intervention coculture group decreased , as well as the positive expression rates of microtubule - associated protein 2 ( MAP -2) and choline acetyltransferase. CONCLUSION; The inflammation mediated by Aβ1-42 inhib their the proliferation of NSCs and induces their apoptosis . Inflammation also significantly reduces the ratio of NSCs differentiating to neu -rons, especially to cholinergic neurons .关键词
小胶质细胞/神经干细胞/阿尔茨海默病/淀粉样β蛋白Key words
Microglial/Neural stem cells/Alzheimer disease/Amyloid beta-protein分类
医药卫生引用本文复制引用
韦美丹,林继宗,朱宁,王克万,王勇..Aβ1-42作用的小胶质细胞对体外培养的神经干细胞生存的影响[J].中国病理生理杂志,2012,28(4):683-688,6.基金项目
国家自然科学基金资助项目 (No.30973162) （No.30973162）
广东省自然科学基金资助项目 (No.07005203) （No.07005203）
