中国病理生理杂志2012，Vol.28Issue(4)：694-699,6.DOI:10.3969/j.issn.1000-4718.2012.04.020
银杏苦内酯B对N-甲基-N-亚硝脲诱导的大鼠视网膜细胞凋亡的抑制作用
Protective mechanisms of ginkgolide B on rat retinal cell apoptosis induced by N-methyl-N-nitrosourea
摘要
Abstract
AIM: To investigate the protective mechanisms of ginkgolide B ( GB) on rat retinal degeneration induced by N - methyl - N - nitrosourea ( MNU). METHODS: The rat retinal degeneration model was made . Photorecep-tor cell apoptosis was measured by TUNEL assay . The expression of Bel - 2 and Bax in the different time points in the rat retina after treated with MNU was measured by RT - PCR and immunohistochemical methods . RESULTS: The outer nuclear layer cell apoptotic index in GB treatment group was significantly lower than that in model group ( P < 0. 01). The bcl -2/bax mRNA ratios at scheduled time points of 12h,ld,2d,3d and 5 d after MNU injection in model control were 0. 36, 0. 15 , 0. 29 , 0. 42 and 0. 64 , respectively , while the ratios in GB group were 0. 98 , 0. 92 , 0. 53 , 0.45 and 0. 68 , respectively, larger than those in model control group (P < 0. 01). No Bcl - 2 positive expression was detected at any scheduled time points after MNU injection in model control group . Strong positive Bcl - 2 expression was detected in GB group 1 d after MNU injection, decreased at the 2nd day and disappeared at the 3 rd day. Compared with model control group , the Bax expression in GB group was significantly decreased (P < 0. 01). CONCLUSION: Ginkgolide B effectively inhibits the apoptosis of photoreceptor cells . The mechanism of GB action may be related to the increase in the expression of Bcl - 2 and the increase in the ratio of Bcl - 2/Bax.关键词
N-甲基-N-亚硝脲/银杏苦内酯B/视网膜/细胞凋亡/光感受器细胞Key words
N-methyl-N-nitrosourea/Ginkgolide B/Retina/Apoptosis/Photoreceptor cells分类
医药卫生引用本文复制引用
孟晶,张旭,陈青山,张日佳,丁小燕..银杏苦内酯B对N-甲基-N-亚硝脲诱导的大鼠视网膜细胞凋亡的抑制作用[J].中国病理生理杂志,2012,28(4):694-699,6.基金项目
广东省医学科研基金资助项目 (No.B2009125) （No.B2009125）
建设中医药强省科研课题(No.2010363) （No.2010363）
广东省科技计划项目(No.2011B031700045) （No.2011B031700045）
