中国病理生理杂志2012，Vol.28Issue(4)：727-729,3.DOI:10.3969/j.issn.1000-4718.2012.04.026
辛伐他汀对香烟烟雾提取物诱导的人脐静脉内皮细胞表达sEPCR和mEPCR的影响
Effects of simvastatin on cigarette smoke extract-induced sEPCR and EPCR expression in human umbilical vein endothelial cells
摘要
Abstract
AIM: To investigate the effects of simvastatin on cigarette smoke extract ( CSE) - induced expres-sion levels of soluble endothelial cell protein C receptor ( sEPCR) and membrane - associated endothelial cell protein C re -ceptor (mEPCR ) in human umbilical vein endothelial cells (HUVECs). METHODS; Cultured HUVECs at passage 4 to 6 were randomly divided into control group , 5 % CSE group, simvastatin groups and simvastatin + CSE groups. In simvastatin groups , HUVECs were incubated with simvastatin at the concentrations of 50, 100 and 200 μmol/L for 24 h. In simvastatin + CSE groups, the cells were treated with simvastatin at the concentrations of 50, 100 and 200 μmol/L for 2 h, and then exposed to CSE for 24 h. The protein level of sEPCR in the culture supernatants was measured by ELISA . The cells were collected for determining the mRNA expression of mEPCR by real - time PCR. RESULTS: Compared with control group, the protein level of sEPCR was significantly increased , and the mRNA expression of mEPCR was significantly de -creased in 5% CSE group ( both P < 0. 05) . The protein levels of sEPCR were significantly increased , and the mRNA expression of mEPCR was significantly decreased in 100 μmol/L and 200 μmol/L simvastatin groups. However, the protein levels of sEPCR were lower , and the mRNA expression of mEPCR was significantly higher in 100 μmol/L and 200 μmol/ L simvastatin groups than those in 5% CSE group. Compared with 5% CSE group, the protein levels of sEPCR in simvastatin + CSE groups were significantly decreased , but higher than those in control group and simvastatin group with correspond -ing concentration. On the contrary, the mRNA expression of mEPCR in simvastatin + CSE groups was significantly increased , but lower than that in control group and simvastatin group with corresponding concentration (all P < 0. 05 ).CONCLUSION: Simvastatin obviously increases the mRNA expression of mEPCR , decreases the protein level of sEPCR , and attenuates the CSE - induced endothelial injury in vitro.关键词
香烟烟雾提取物/内皮细胞蛋白C受体/人脐静脉内皮细胞Key words
Cigarette smoke extract/Endothelial cell protein C receptor/Human umbilical vein endothelial cells分类
医药卫生引用本文复制引用
樊芳芳,胡晓芸,扈丽娟,韩葆芬,赵瑛娟..辛伐他汀对香烟烟雾提取物诱导的人脐静脉内皮细胞表达sEPCR和mEPCR的影响[J].中国病理生理杂志,2012,28(4):727-729,3.基金项目
山西省卫生厅科技攻关计划项目(No.20100201) （No.20100201）
