中国病理生理杂志2012，Vol.28Issue(5)：785-790,6.DOI:10.3969/j.issn.1000-4718.2012.05.003
硫唑嘌呤对大鼠骨髓间充质干细胞增殖、细胞周期和凋亡的影响
Effects of azathioprine on proliferation, cell cycles and apoptosis of me senchymal stem cells from rat bone marrow in vitro
摘要
Abstract
AIM: To invesligale the effects of azathioprine ( AZA) on the proliferation, cell cycle and apoplo-sis of mesenchymal stem cells (MSCs) from the bone marrow of Sprague - Dawley rats in vitro. METHODS; MSCs were cullured in low - glucose DMEM containing 10% FBS,and trealed with AZA at concenlralions of 50 mg/L, 100 mg/L, 200 mg/L and 300 mg/L for 24 h, 48 h and 72 h. The effecls of AZA on the growlh curve and proliferation of MSCs were tesled by cell counler under microscope. The apoplosis and cell cycle was determined by flow cylomelry. RESULTS; Pure MSCs were gained by 3 limes of passages. No significant effecl of AZA at concentration of less than 100 mg/L on the proliferation, cell cycle and apoptosis of MSCs was observed (P > 0. 05). Under the condition of more than 200 mg/L for 72 h, AZA inhibited the growth of MSCs. The inhibitory rate was more than 66% , and the rate of apoptosis was increased (P < 0. 05). However, at the concentration of 300 mg/L for 72 h, AZA decreased the apoptotic rate and the necrotic rate of MSCs was obviously increased (P <0. 05). Using AZA at concentration of more than 200 mg/L, as the action time prolonged , the MSCs in G0/G1 phase were increased, and those in S phase were decreased ( P < 0. 05 ) . CONCLUSION: At some concentrations, AZA significantly affects the proliferation, apoptosis and cell cycle of MSCs. Large dose of AZA may cause MSCs to death.关键词
骨髓间充质干细胞/细胞凋亡/细胞周期/硫唑嘌呤/大鼠Key words
Bone marrow mesenchymal stem cells/ Apoptosis/ Cell cycle/ Azalhioprine/ Rats分类
医药卫生引用本文复制引用
昝慧,黄花荣,钟英强,吴新环,叶小研..硫唑嘌呤对大鼠骨髓间充质干细胞增殖、细胞周期和凋亡的影响[J].中国病理生理杂志,2012,28(5):785-790,6.基金项目
中国医疗手牵手工程委员会、北京医学奖励基金会(No.XHYSGZZSDX-001) （No.XHYSGZZSDX-001）
