中国病理生理杂志2012，Vol.28Issue(5)：834-838,5.DOI:10.3969/j.issn.1000-4718.2012.05.012
盐霉素抑制人肺腺癌耐顺铂细胞株A549/DDP增殖及诱导凋亡的机制
Salinomycin inhibited proliferation and induced apoptosis of cisplatin-resistant human lung adenocarcinoma cell line A549/DDP
曾葭 1刘成成 2祝爱珍 2陈小宇 2谭广销 2刘革修2
作者信息
- 1. 中国人民解放军411医院呼吸内科,上海,200081
- 2. 暨南大学医学院血液病研究所,广东,广州,510632
- 折叠
摘要
Abstract
AIM: To invesligale the effect of salinomycin on the proliferation and apoptosis of cisplalin - resist-ant human lung adenocarcinoma cell line A549/DDP. METHODS: The inhibitory effect of salinomycin on the growlh of A549/DDP cells was tesled by MTT method in vitro. The apoptosis and milochondrial membrane potential ( △ψm ) of A549/DDP cells were assayed by flow cylomelry. The aclivily of caspase -3,8 and 9 was determined by the melhod of col-orimeLry. The levels of cylochrome C, Bcl - 2 , Bax, β - catenin, and phosphorylaled low - densily lipoprolein receptor -relaled protein 6(p - LRP6) were measured by Weslern blotting. RESULTS: Salinomycin inhibited the growth of A549/ DDP cells in a dose - dependent manner. Salinomycin at concentration of 0. 2 μmol/L decreased △ψm level, and increased reactive oxygen species (ROS) , cytochrome C and cytosolic Ca2+release in the cells. Salinomycin also increased the activity of caspase -3,8, and 9 in the cells, reduced the ratio of Bcl - 2/Bax, and decreased the levels of β - catenin and p- LRP6. CONCLUSION: Salinomycin depresses the cell growth by inhibiting Wnt signaling, and induces the apoptosis of cisplatin - resistant human lung adenocarcinoma cell line A549/DDP via mitochondria - dependent and Bcl - 2/Bax pathways.关键词
盐霉素/肺肿瘤/顺铂/细胞凋亡/β-catenin/低密度脂蛋白受体相关蛋白6Key words
Salinomycin/ Lung neoplasms/ Cisplatin/ Apoptosis/ β -catenin/ Low - density lipoprotein receptor - related protein 6分类
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曾葭,刘成成,祝爱珍,陈小宇,谭广销,刘革修..盐霉素抑制人肺腺癌耐顺铂细胞株A549/DDP增殖及诱导凋亡的机制[J].中国病理生理杂志,2012,28(5):834-838,5.
