中国病理生理杂志2012，Vol.28Issue(5)：839-845,7.DOI:10.3969/j.issn.1000-4718.2012.05.013
胡椒碱减轻H2O2引起的兔心房肌细胞内向整流钾电流及超速激活延迟整流钾电流的异常改变
Effects of piperine on abnormalities of inward rectifier potassium current and ultra rapid delayed rectifier potassium current induced by hydrogen peroxide in rabbit atrial myocytes
摘要
Abstract
AIM: To study the proleclive effect of piperine on abnormalilyies of inward rectifier potassium cur-rent ( IK1 ) and ultra rapid delayed rectifier polassium currenl ( IKUT) induced by hydrogen peroxide ( H2 O2 ) in single rabbil alrial myocytes. METHODS: The technique of whole - cell patch - clamp was used Lo study the effecl of H2 O2 at concen-tralion of 50 μmol/L on IK1 and /Kut in single rabbil alrial myocyles. The proleclive effecl of prelrealmenl with piperine (7 μmol/L) was also observed. RESULTS:The piperine al concenlralion of 7 μmol/L had no significant effecl on IK1 and IKUt and their channel dynamics. In the presence of H2O2 at concenlralion of 50 μmol/L, the peak currents of /K1 and /KUT reduced significanlly ( P < 0. 05 ) . The sleady - state aclivalion curve of /Kut was shifted right, the sleady - state inaclivalion curve of IKUt was shifted left, and the recovery from inaclivalion of IKUt was shifted downward. The IKUt showed frequency -dependenl characteristics. Piperine al concenlralion of 7 μmol/L significanlly alleviated the inhibitory effecl of H2O2 on IK1 and IKUt(P <0. 01). In addition, piperine prolected againsl the changes of IKUt dynamics induced by H2O2. CONCLUSION : Piperine alleviates the abnormalities of IK1 and IKUt induced by oxidative slress in alrial myocyles.关键词
胡椒碱/过氧化氢/心房肌细胞/内向整流钾电流/超速激活延迟整流钾电流Key words
Piperine/ Hydrogen peroxide/ Alrial myocyles/ Inward rectifier polassium current/ Ullra rapid delayed rectifier polassium current分类
医药卫生引用本文复制引用
刘岩,李泱,林琨,田苗,王玉堂,单兆亮..胡椒碱减轻H2O2引起的兔心房肌细胞内向整流钾电流及超速激活延迟整流钾电流的异常改变[J].中国病理生理杂志,2012,28(5):839-845,7.基金项目
国家自然科学基金资助项目(No.30772886) （No.30772886）
