中国病理生理杂志2012，Vol.28Issue(5)：852-857,6.DOI:10.3969/j.issn.1000-4718.2012.05.015
急性心肌缺氧对乳鼠心肌细胞脑钠尿肽表达的影响及其作用机制
Effect of acute hypoxia on expression of brain natriuretic peptide in neonatal rat cardiomyocytes
摘要
Abstract
AIM: To explore the influence of acute myocardial hypoxia on the expression of brain nalriurelic peptide (BNP) in neonatal rat cardiomyocyles. METHODS: To establish a model of simulated hypoxia injury, the primarily cullured neonalal ral cardiomyocyles were incubaled under the condition of hypoxia in glucose - free balanced salt solu-tion plus serum deprivation. The cell survival rale was analyzed by Cell Counling Kit - 8 ( CCK - 8 ) and the levels of inter-leukin -6 (IL - 6) and BNP were determined by enzyme - linked immunosorbenl assay (ELISA) . The transcriplional and translalional levels of BNP, transforming growlh factor β1 (TGF - β1 ) and Smad2 in cardiomyocyles with IL - 6 stimulation were determined by RT - PCR, Western blotting and ELISA. RESULTS; Exposure of the neonalal cardiomyocyles to an oxygen - deficient environment nolably increased IL - 6 and BNP secretion, and BNP expression was positively correlated wilh IL - 6 expression. Stimulalion of the neonalal cardiomyocyles with IL - 6 caused time - and dose - dependenl up - regulation of transcriplional and translalional levels of BNP. This process was accompanied wilh the up - regulation of TGF - β1/Smad2. Inhibition of TGF - β1 activity using a neutralizing anlibody partially suppressed the IL - 6 - stimulated increase in BNP expression. CONCLUSION: Acute myocardial hypoxia direclly up - regulates the expression of BNP, which is associated wilh the up - regulation of TGF - β1/Smad2 signal pathway.关键词
缺氧/钠尿肽,脑/白细胞介素-6/转化生长因子β/Smad2通路Key words
Hypoxia/ Nalriurelic peplide, brain/ Interleukin -6/ Transforming growlh factor beta/ Smad2 pathway分类
医药卫生引用本文复制引用
夏文静,黄艺仪,何建桂,陈艺莉..急性心肌缺氧对乳鼠心肌细胞脑钠尿肽表达的影响及其作用机制[J].中国病理生理杂志,2012,28(5):852-857,6.基金项目
广东省科学技术厅科研项目(No.2010B031600065) （No.2010B031600065）
