中国病理生理杂志2012，Vol.28Issue(5)：884-888,5.DOI:10.3969/j.issn.1000-4718.2012.05.020
人膝骨关节炎血清生物学标志物的筛选
Analysis of serum biomarkers in human knee osteoarthritis
摘要
Abstract
AIM: To analyze the proleomic componenls of the sera from knee osleoarthrilis patients and normal people, and to search proleins that might serve as serum biomarkers for osleoarlhrilis diagnosis, trealmenl or palhogenesis. METHODS: Two -dimensional fluorescence difference gel eleclrophoresis (2D - DIGE) was applied lo analyze the sera oblained from the palienls with knee osleoarlhrilis (n =4) and normal controls (n =4). The differentially expressed proleins were idenlified by malrix - assisted laser desorplion ionizalion lime - of - flighl mass speclromelry ( MALDI - TOF -MS). Western blotting analysis was applied lo confirm the resulls. RESULTS; Comparalive proleomic data of serum from the palienls wilh osteoarlhrilis was successfully oblained. Eight differentially expressed prolein spots were observed. Five up - regulaled and 3 down - regulated proleins were identified. Weslern blot analysis confirmed that α2 - macroglobulin was increased. CONCLUSION: There are significant differences between serum proleins obtained from the patienls with knee osleoarlhrilis and normal controls. Α2 - Macroglobulin might be utilized as potenlial biomarkers for the diagnosis and -realmenl of osteoarlhrilis.关键词
骨关节炎/血清/生物学标记物/双向荧光差异凝胶电泳Key words
Osleoarlhrilis/ Serum/ Biomarkers/ Two - dimensional fluorescence difference gel eleclrophoresis分类
医药卫生引用本文复制引用
曾花,蔡道章,陈郁鲜,曾春,叶志强,庄泽,黎明涛,胡坤华..人膝骨关节炎血清生物学标志物的筛选[J].中国病理生理杂志,2012,28(5):884-888,5.基金项目
国家高技术研究发展计划项目(No.2008AA02Z437) （No.2008AA02Z437）
