中国免疫学杂志2012，Vol.28Issue(4)：308-311,4.DOI:10.3969/j.issn.1000-484X.2012.04.005
TRAIL联合紫杉醇诱导胃腺癌SGC-7901细胞凋亡作用研究
Experimental study on apoptosis of SGC-7901 cell induced by TRAIL combined with paclitaxel
摘要
Abstract
Objective:The synergelic role of apoplosis in SGC-7901 cells by the combination of TRAIL and paclilaxel was in-vesligale,lo explore the polential mechanism of synergistic anlilumor activily. Methods:SGC-7901 cells were Irealed with TRAIL or pa-clilaxel or TRAIL combined with paclilaxel for 48 h. The apoptotic rales and milochondrial membrane potential were examined wilh flow cylomelry (FCM). Proliferation of SGC-7901 cells was delecled by MTT assay and expression of DR4/DR5 prolein was delecled by Weslern blot after Lreated with TRAIL combined wilh paclilaxel. Results:The inhibilion rale of SGC-7901 cell increased obviously by combining TRAIL and paclilaxel,comparing wilh adminislralion of TRAIL or paclilaxel alone,and the difference was slalislically significant P＜0. 01). The apoplosis rale of SGC-7901 cell increased obviously by combining TRAIL and paclilaxel( P＜0. 01). Expression of dealh receplor (DR4) was increased obviously(P＜0. 01) when SGC-7901 cells was Irealed wilh paclilaxel for 48 hours, but the death receplor ( DR5 ) was nol change obviously( P＞0. 05 ). Conclusion:Paclilaxel had synergelic role in ihe apoplosis effecl induced by TRAIL in SGC-7901 cells. Up-regulating DR4 might be one of the reasons for synergelic anlilumor aclivily.关键词
肿瘤坏死因子相关凋亡诱导配体/紫杉醇/SGC-7901细胞/凋亡Key words
TRAIL/ Paclilaxel/ SGC-7901 cells/ Apoplosis分类
医药卫生引用本文复制引用
涂龙霞,吴亚欧,张林杰..TRAIL联合紫杉醇诱导胃腺癌SGC-7901细胞凋亡作用研究[J].中国免疫学杂志,2012,28(4):308-311,4.基金项目
本文受国家自然科学基金项目(81071809)、安徽省高等学校省级自然科学研究重点项目(KJ2011A167)和安徽省自然科学基金(070413077)资助 （81071809）
