中国免疫学杂志2012，Vol.28Issue(4)：312-315,322,5.DOI:10.3969/j.issn.1000-484X.2012.04.006
靶向沉默Ku80增强顺铂诱导的人肺腺癌细胞凋亡
Targeted reduction of ku80 enhanced cisplatin-induced apoptosis in lung adenocarcinoma cells
摘要
Abstract
Objective:To invesligale the polential of Ku80 small-interfering RNA (siRNA) Lo enhance cisplalin-induced apop-Losis. Methods:Ku80 mRNA and prolein expression levels were investigaled by reverse transcription-PCR and Western blot analysis,re-spectively. Ku80-specific siRNA was synthesized and Lransfected inlo cisplatin-resistant human lung adenocarcinoma cell line A549/ DDP prior Lo LreaLment with cisplalin. Cell survival was assessed by MTT assay. Apoptosis was assessed using flow cylomelry analysis. Caspase-3 activity was detected by speclropholomeler. Results:The Ku80 mRNA and prolein expressions were significantly elevaled in A549/DDP cells compared wilh the parenlal A549 cells. The Ku80 mRNA and prolein expressions were significantly downregulaled by si-Ku80 in A549/DDP cells. Treated wilh cisplalin for 24 h, the inhibilion rales of si-Ku80 cells decreased significantly compared wilh in conlrol group and si-Scramble group. After 24 h trealmenl wilh 6 μg/ml cisplalin, apoplosis rale in si-Ku80 group was increased compared wilh the conlrol group and si-Scramble group ( P＜0. 05 ) ; aclivilies of Caspase-3 significantly increased in siKu80-A549/ DDP cells as compared wilh conlrol cells and si-Scramble cells (P＜0. 01). Conclusion:Ku80 may correlate wilh the chemoresislance lo cisplalin in lung cancer. Ku80-specific siRNA enhanced cisplalin-induced apoplosis in the cisplalin-resislanl A549/DDP cells.关键词
肺腺癌/耐药/顺铂/Ku80/siRNA/凋亡Key words
Lung adenocarcinoma/ Drug resistance/ Cisplalin/ Ku80/ siRNA/ Apoplosis分类
医药卫生引用本文复制引用
黎萍,许溟宇,马青山,张捷..靶向沉默Ku80增强顺铂诱导的人肺腺癌细胞凋亡[J].中国免疫学杂志,2012,28(4):312-315,322,5.基金项目
本文为吉林省科技发展计划项目(2007084)资助项目 （2007084）
