中国免疫学杂志2012，Vol.28Issue(4)：333-337,5.DOI:10.3969/j.issn.1000-484X.2012.04.010
人母-胎界面滋养细胞来源的CXCL16促进蜕膜γδT细胞产生IL-10及TGF-β
摘要
Abstract
Objective:To study the effect of human trophoblasts on the production of IL-10 and TGF-β in decidual γδT cells via the CXCL16/CXCR6 pathway. Methods:10 samples of villus and deciduals were collected from normal early pregnant women, then we isolated the trophoblasts and decidual γδT cells respectively. We detected the concentration of CXCL16 in the supernatant of trophoblasts by ELISA and the CXCR6 mRNA of decidual γδT cells by RT-PCR. We established the co-culture system of trophoblasts and decidual γδT cells, then detected the expression of intracellular cytokines IL-10 and TGF-β in the γδT cells by FCM. Results:The trophoblasts from early pregnant women could continually secrete CXCL16 in vitro, with a time-dependent effect, and decidual γδT cells could express CXCR6. In the direct co-culture condition, the trophoblasts significantly promoted the production of IL-10 and TGFβ in decidual γδT cells. This stimulatory effect of trophoblasts was partially abolished by CXCL16 neutralizing antibody. However, when decidual γδT cells were indirectly cocultured with trophoblasts, the production of IL-10 and TGF-β in decidual γδT cells was similar to the control. Conclusion:Human trophoblasts might promote IL-10 and TGF-β production in decidual γδT cells via CXCL16/ CXCR6 pathway, which is beneficial to the Th2 bias phenomenon at maternal-fetal interface and maintaining maternal immunotoleranee toward the fetus in early human pregnancy.关键词
滋养细胞/γδT细胞/IL-10/TGF-β/CXCL16/CXCR6Key words
Trophoblast/γδT cell/IL-10/TGF-β/CXCLl/CXCR6分类
医药卫生引用本文复制引用
王莹,范登轩,李大金,金莉萍..人母-胎界面滋养细胞来源的CXCL16促进蜕膜γδT细胞产生IL-10及TGF-β[J].中国免疫学杂志,2012,28(4):333-337,5.基金项目
本文为国家自然科学基金(No.31170870)、教育部博士点新教师基金(No.200802461019 )和上海市自然科学基金(No.10ZR1405100) （No.31170870）
