中国免疫学杂志2012，Vol.28Issue(4)：347-350,4.DOI:10.3969/j.issn.1000-484X.2012.04.014
早期诊断结核性脑膜炎的新方法:检测浓缩脑脊液中CFP-10和ESAT-6
Early diagnosis of tuberculous meningitis new methods: detection concentration in cerebrospinal fluid CFP-10 and ESAT-6
摘要
Abstract
Objective:Using ELISA to detect the Mycobacterium tuberculosis culture filtrate protein 10(CFP10) and early secretory target antigen 6000(ESAT-6) in CSF, assess its diagnosing value of the early diagnose of tubercular meningitis (TBM) . Metll-odS;The CSF of 102 patients was collected,46 of them were clinic diagnosed as TBM, 56 of them were not. Using specific antigen ELISA kit to detect CFP10 and ESAT-6 in the cerebrospinal fluid stoste and the dialysis bag enrichment ten times of cerebrospinal fluid concentrate, respectively. Results:Cerebrospinal fluid stoste CFP10 detection TBM sensitivity was 13.04%, specific degrees for 100. 00% , ESAT-6 of sensitivity was 13. 04% , specific degrees of 100. 00% . Concentrated Cerebrospinal fluid, the sensitivity of CFP10 was 78. 26% , specificity of it was 96.42%; the sensitivity and specificity of ESAT-6 were 76.09% and 98. 18%. Conclusions: Using the dialysis to concentrate samples can increase the sensitivity of using specific antigen ELISA to detect the CFP10 and ESAT-6 in the CSF, it is a brief and fast method to diagnose TBM, also has great meaning in the early diagnosis of TBM.关键词
结核性脑膜炎/酶联免疫吸附试验/CFP10/ESAT-6Key words
Tubercular meningitis/ Enzyme-linked immunoabsorbent assay/ CFP10/ESAT-6分类
医药卫生引用本文复制引用
吴晓牧,刘丽娟,许志强,胡国柱,曹文锋,黄刚,胡凡,张昆南..早期诊断结核性脑膜炎的新方法:检测浓缩脑脊液中CFP-10和ESAT-6[J].中国免疫学杂志,2012,28(4):347-350,4.基金项目
本文为江西省科技厅资助项目(20094001)和江西省卫生厅资助项目(2010BSA11300) （20094001）
