中国免疫学杂志2012，Vol.28Issue(4)：357-361,5.DOI:10.3969/j.issn.1000-484X.2012.04.017
白细胞介素23在哮喘小鼠动物模型中表达的研究
The expression of interleukin 23 (IL-23) in model of asthmatic mice
李艳春 1孙萌 2赵丽娜 3刘丽 1鲁继荣1
作者信息
- 1. 吉林大学第一医院小儿呼吸一科,长春,130021
- 2. 青岛市妇女儿童医疗保健中心感染科,青岛,266011
- 3. 吉林大学中日联谊医院甲状腺外科,长春,130033
- 折叠
摘要
Abstract
Objective:To study the expression of IL-23 in model of asthma in asthmatic mice. Methods:Mouse models were established and divided into two groups, normal control group and asthma group. The levels of IL-23 in serum were detected by ELISA, the levels of IL-23pl9mRNA and IL-23pl9 protein in lung tissue were detected respectively by RT-PCR and immunohistochemistry. Spleen T lymphocytes were isolated and cultivated in vitro. The levels of IL-23pl9mRNA and IL-23pl9 protein in T lymphocytes were detected respectively by RT-PCR and Western blot. Results:Compare to normal control group, the level of IL-23 in serum was higher in asthma group (P＜0. 01) , the levels of IL-23pl9mRNA and IL-23pl9 protein in lung tissues were higher in asthma group(P＜0. 01) , and the levels of IL-23pl9mRNA and IL-23pl9 protein in spleen T lymphocytes was higher in asthma group(P＜0. 01) . Conclusion: IL-23 may involve in the pathogenesis of asthma, and may have influence on T lymphocytes.关键词
白细胞介素23/支气管哮喘/动物模型Key words
Interleukin 23 / Asthma/ Animal model分类
医药卫生引用本文复制引用
李艳春,孙萌,赵丽娜,刘丽,鲁继荣..白细胞介素23在哮喘小鼠动物模型中表达的研究[J].中国免疫学杂志,2012,28(4):357-361,5.
