中国免疫学杂志2012，Vol.28Issue(4)：368-371,383,5.DOI:10.3969/j.issn.1000-484X.2012.04.020
肿瘤患者胸/腹水和外周血中TCR Vβ亚家族表达及调节性T细胞亚群的研究
Study on the T cells receptor (TCR) Vβ repertoire and the expression profile of Treg between peripheral blood mononuclear cells and the tumor infiltrating lymphocytes (TIL) in patients with carcinoma
摘要
Abstract
Objective:To analyze the T cells receptor (TCR) Vβ repertoire, CD4/CD8 ratio, and the expression profile of Treg between peripheral blood mononuclear cells and the tumor infiltrating lymphocytes (TIL) in patients with carcinoma. Methods: cells were isolated from the pleural effusion or ascites of carcinoma patients, and from the peripheral blood of carcinoma patients and healthy individuals. The expressions of 24 TCR Vβ subfamilies, CD3, CD4, CD8, CD25 , and CD127 were detected by flow cytometry (FCM) . The T cells receptor (TCR) Vβ repertoire, CD4/CD8 ratio, and Treg expression profile were statistically analyzed. Results: There was a distinct difference in TCR Vβ repertoire between TILs from pleural effusion/as cites and T cells from peripheral blood in patients with carcinoma. TCR Vβ8 in TILs of lung carcinoma patients (4/5) and TCR Vβ2 in TILs of colon carcinoma patients (3/4) showed higher proportion than that in peripheral blood T cells of patients and healthy controls. The proportion of Treg was higher in pleural effusion/ as cites than in peripheral blood of carcinoma patients or healthy controls (P＜0. 05) . Correspondingly, the proportion of CD8 + T cells was lower in pleural effusion than in peripheral blood in 5 lung carcinoma patients. Conclusion:The distinct differences in TCR repertoire between TILs and peripheral blood T cells imply that selective expansion and chemotaxis of T cells have occurred in carcinoma patients. In addition, the tumor microenvironment may affect the differentiation of CD4+ T cells and lead to the increased Treg and decreased CD8+ T cells in pleural effusion/ascites, which may also affect the selective expansion of TILs and led to immune tolerance.关键词
肿瘤/TCR Vβ表达谱/调节性T细胞Key words
Carcinoma/ TCR Vβ repertoire/ Regulatory T cells分类
医药卫生引用本文复制引用
区裕升,邵红伟,吴凤麟,黄树林,肖兰凤..肿瘤患者胸/腹水和外周血中TCR Vβ亚家族表达及调节性T细胞亚群的研究[J].中国免疫学杂志,2012,28(4):368-371,383,5.基金项目
本文受广东省重大新药创制科技重大专项( 2009ZX09103-708)、国家自然科学基金(31100664)、广东省自然科学基金 (10151022401000024)和广东药学院师资队伍建设经费资助 （ 2009ZX09103-708）
