中国农业科学2012，Vol.45Issue(1)：44-51,8.DOI:10.3864/j.issn.0578-1752.2012.01.006
细胞分裂素对水稻分蘖芽生长及分蘖相关基因表达的调控
Effect of Cytokinins on the Growth of Rice Tiller Buds and the Expression of the Genes Regulating Rice Tillering
摘要
Abstract
[Objective] The objective of the present study was to investigate the relationship of cytokinins (CTK) and the expression of the genes regulating rice tillering during the regulating the growth of rice tiller buds growth and the underlying molecular mechanisms. [Method] In the present study, a cultivar Nanjing 44 was used. At tillering stage, 40 mg·L-1 nitrogen (N) was used to stimulate the germination of tiller buds. At the same time, external IAA was used to inhibit the germination of tiller buds. The changes of CTK contents in tiller buds and tiller nodes and the expression of OsTB1, OsD3, OsD10 and OsD27 in tiller buds were measured. [Result] The external 40 mg-L-1 N increased the CTK contents in tiller nodes and tiller buds, inhibited the expression of OsTB1, OsD3, OsD10 and OsD27 in tiller buds, and then promoted the growth of rice tiller buds. However, the external application of IAA reversed the effect of N. Besides this, the expression of OsTBl in tiller buds was regulated by CTK, however, the expression of OsD3, OsDIO and OsD27 in tiller buds might not be regulated by CTK. [Conclusion] The results showed that external N and IAA might have regulated tiller bud growth by at least two means: one is regulating the CTK content and the expression level of OsTBl in tiller buds, another is regulating the expression level of OsD3, OsD10 and OsD27 in tiller buds and by this to regulate the tiller bud growth.关键词
水稻/细胞分裂素/分蘖芽/分蘖相关基因Key words
rice/cytokinin/tiller bud/genes regulating tillering引用本文复制引用
刘杨,顾丹丹,许俊旭,丁艳锋,王强盛,李刚华,刘正辉,王绍华..细胞分裂素对水稻分蘖芽生长及分蘖相关基因表达的调控[J].中国农业科学,2012,45(1):44-51,8.基金项目
国家自然科学基金项目(31071364)、江苏省自然科学基金项目(BK2010449) （31071364）
